{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:12:39Z","timestamp":1792033959875,"version":"4.4.0"},"reference-count":13,"publisher":"National Academy of Sciences","issue":"17","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI145939"],"award-info":[{"award-number":["R01AI145939"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI159583"],"award-info":[{"award-number":["R21AI159583"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,4,28]]},"abstract":"<jats:p>\n                    The recently emergent fungal pathogen\n                    <jats:italic toggle=\"yes\">Candidozyma (Candida) auris<\/jats:italic>\n                    has produced numerous outbreaks of invasive disease in hospitals worldwide. It is the first fungal pathogen categorized as a global public health threat due to its ability to readily colonize skin, spread efficiently person-to-person, and cause invasive infection with high mortality. Skin colonization not only promotes healthcare spread but also leads to invasive infection via the insertion of medical devices through skin. However, the mechanisms underlying\n                    <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                    survival in the skin niche remain poorly understood. Here, we identify potassium (K\n                    <jats:sup>+<\/jats:sup>\n                    ) as a crucial nutrient for efficient growth in this environment. We show that putative high-affinity K\n                    <jats:sup>+<\/jats:sup>\n                    transporter Trk1 is required for\n                    <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                    growth on human skin ex vivo. Disruption of\n                    <jats:italic toggle=\"yes\">TRK1<\/jats:italic>\n                    significantly impairs K\n                    <jats:sup>+<\/jats:sup>\n                    uptake and leads to plasma membrane hyperpolarization, a lower intracellular pH, and sensitivity to cationic stress. These findings demonstrate that\n                    <jats:italic toggle=\"yes\">TRK1<\/jats:italic>\n                    is necessary for maintaining K\n                    <jats:sup>+<\/jats:sup>\n                    homeostasis during\n                    <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                    colonization of human skin, shedding light on pathogenesis for this emerging threat. Given that\n                    <jats:italic toggle=\"yes\">TRK1<\/jats:italic>\n                    has no known human homologs, the gene product may serve as a promising therapeutic target for development of novel strategies directed at\n                    <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                    propagating on skin.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2602824123","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T16:25:41Z","timestamp":1776788741000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":5,"title":["Trk1 potassium transport is crucial for effective\n                    <i>Candidozyma auris<\/i>\n                    skin colonization"],"prefix":"10.1073","volume":"123","author":[{"given":"Adam J.","family":"Glawe","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily F.","family":"Eix","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Wisconsin-Madison","place":["Madison"]},{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Zarnowski","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maisy K.","family":"Andes","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-9100","authenticated-orcid":false,"given":"James","family":"Lazarcik","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5295-2121","authenticated-orcid":false,"given":"Katherine A.","family":"Henzler-Wildman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8587-6244","authenticated-orcid":false,"given":"Jeniel E.","family":"Nett","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Wisconsin-Madison","place":["Madison"]},{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin-Madison","place":["Madison"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw691"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myz112"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00910-19"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2020.183486"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153374"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00042-09"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/23328940.2019.1632145"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2144-8_9"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5199"},{"key":"e_1_3_3_10_2","first-page":"497","article-title":"Potassium deprivation is sufficient to induce a cell death program in Saccharomyces cerevisiae","volume":"10","author":"Lauff D. B.","year":"2010","unstructured":"D. B. Lauff, G. E. Santa-Maria, Potassium deprivation is sufficient to induce a cell death program in Saccharomyces cerevisiae. FEMS Yeast Res. 10, 497\u2013507 (2010).","journal-title":"FEMS Yeast Res."},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M806693200"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adf8972"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiac094"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2602824123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T16:26:29Z","timestamp":1776788789000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2602824123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,21]]},"references-count":13,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2026,4,28]]}},"alternative-id":["10.1073\/pnas.2602824123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2602824123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,21]]},"assertion":[{"value":"2026-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2602824123"}}