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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,7,28]]},"abstract":"<jats:p>\n                    Adhesion to tissues and surfaces is used by pathogens to avoid clearance, maintain infections, and facilitate transmission. An important example is the adhesion of the\n                    <jats:italic toggle=\"yes\">Leishmania<\/jats:italic>\n                    parasite to the stomodeal valve of its insect vector, the sand fly, which is important for life cycle progression, and occurs through its flagellum via a cytoskeletal adhesion complex. While three essential kinetoplastid-insect adhesion proteins (KIAPs) are known, the overall proteome of the adhesion complex is obscure. Using TurboID-tagged KIAP3 combined with proteomics and light microscopy, we have identified additional proteins associated with the adhered flagellum, including multiple members of the Adhesion Related NTPase-like Domain (ARND) family, of which the canonical member is KIAP4, described here. Phylogenetic analysis of the ARND family showed it is conserved across the kinetoplastids, and we demonstrate that\n                    <jats:italic toggle=\"yes\">Trypanosoma congolense<\/jats:italic>\n                    paralogs localize to the adhered flagellum in that parasite. Deletion of KIAP4 severely impaired haptomonad adhesion, with the mutant parasites unable to colonize the sand fly stomodeal valve. Our work provides details of the molecular machinery essential for vector colonization and shows that these proteins have a conserved function in other kinetoplastid parasites.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2603653123","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T19:15:07Z","timestamp":1784574907000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1,"title":["Identification of a conserved gene family with an essential role in\n                    <i>Leishmania<\/i>\n                    parasite\u2013insect vector adhesion"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9469-399X","authenticated-orcid":false,"given":"Barrack O.","family":"Owino","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v2twj65","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological and Medical Sciences, Oxford Brookes University","place":["Oxford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9419-398X","authenticated-orcid":false,"given":"Ryuji","family":"Yanase","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v2twj65","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological and Medical Sciences, Oxford Brookes University","place":["Oxford, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/01ee9ar58","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Life Sciences, University of Nottingham Medical School","place":["Nottingham, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1350-4251","authenticated-orcid":false,"given":"Katerina","family":"Pruzinova","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024d6js02","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Parasitology, Charles University, Faculty of Science","place":["Prague, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Farr","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"name":"Sir William Dunn School of Pathology, University of Oxford","place":["Oxford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1889-9985","authenticated-orcid":false,"given":"Yaimie","family":"Lopez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v2twj65","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological and Medical Sciences, Oxford Brookes University","place":["Oxford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2606-5641","authenticated-orcid":false,"given":"Alan O.","family":"Marron","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v2twj65","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological and Medical Sciences, Oxford Brookes University","place":["Oxford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Vaughan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v2twj65","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological and Medical Sciences, Oxford Brookes University","place":["Oxford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1790-1123","authenticated-orcid":false,"given":"Petr","family":"Volf","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024d6js02","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Parasitology, Charles University, Faculty of Science","place":["Prague, Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2836-9622","authenticated-orcid":false,"given":"Jack D.","family":"Sunter","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v2twj65","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological and Medical Sciences, Oxford Brookes University","place":["Oxford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,7,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.158"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","unstructured":"K. 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