{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:31:09Z","timestamp":1786113069018,"version":"build-2736575974"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of wuhan","award":["2025040601020217"],"award-info":[{"award-number":["2025040601020217"]}]},{"name":"Fundamental research funds for the central university","award":["YCJJ20252432"],"award-info":[{"award-number":["YCJJ20252432"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,8,11]]},"abstract":"<jats:p>\n                    Dendritic cell (DC) vaccines offer professional antigen presentation but are often limited by suboptimal trafficking to lymphoid tissues, whereas whole tumor cell vaccines preserve antigenic diversity yet frequently lack coordinated activation cues for efficient T cell priming. Here, we report OncoAPC, an inactivated artificial antigen-presenting cell designed around a triple-signal priming logic: MHC-I-mediated antigen presentation (signal 1), CD80-mediated costimulation (signal 2) with checkpoint-insulating capacity, and incorporated IL-12 (signal 3). Mechanistically, OncoAPC directly activated T cells while partially insulating against PD-1 suppression through\n                    <jats:italic toggle=\"yes\">cis<\/jats:italic>\n                    CD80:PD-L1 interactions and simultaneously engaged endogenous DCs to relay tumor antigens via cross-dressing and amplify lymphoid priming. Across multiple tumor models, OncoAPC showed broad therapeutic activity, including marked suppression of metastatic tumor burden and substantial control of established tumors, outperforming conventional DC vaccination. It further retained efficacy beyond the MC38 backbone and remained active when generated from human tumor cells or excised tumor-derived material. These findings establish OncoAPC as a scalable, broad-spectrum cancer vaccine platform that integrates antigenic diversity with DC-like functional precision.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2604171123","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T17:36:00Z","timestamp":1785951360000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Checkpoint-insulated triple-signal artificial antigen-presenting cells drive antigen relay and systemic antitumor immunity"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0283-4730","authenticated-orcid":false,"given":"Siyu","family":"Zhao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00p991c53","id-type":"ROR","asserted-by":"publisher"}],"name":"Tongji School of Pharmacy, Huazhong University of Science and Technology","place":["Wuhan, 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Zhao Data from \u201cBulk RNA-seq profiling of MC38 subcutaneous tumors after OncoAPC treatment.\u201d NCBI Sequence Read Archive. https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1469797. Deposited 22 May 2026."},{"key":"e_1_3_4_51_2","unstructured":"S. Zhao Data from \u201cSingle-cell RNA sequencing of OncoAPC-treated tumors.\u201d NCBI Sequence Read Archive. https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1478197. Deposited 13 June 2026."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2604171123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T17:36:17Z","timestamp":1785951377000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2604171123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,5]]},"references-count":51,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2026,8,11]]}},"alternative-id":["10.1073\/pnas.2604171123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2604171123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,5]]},"assertion":[{"value":"2026-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2604171123"}}