{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:35:44Z","timestamp":1786995344654,"version":"build-2736575974"},"reference-count":53,"publisher":"National Academy of Sciences","issue":"34","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["AST 1678424"],"award-info":[{"award-number":["AST 1678424"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["F7916-B"],"award-info":[{"award-number":["F7916-B"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["2034021"],"award-info":[{"award-number":["2034021"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:p>\n                    Thylakoid membranes are indispensable for oxygenic photosynthesis, yet the mechanisms that protect these membranes from photooxidative damage remain poorly understood. By screening poorly characterized proteins induced during the chloroplast unfolded protein response, we identify VIA1 as an essential factor for preserving thylakoid integrity under high light in the model green alga\n                    <jats:italic toggle=\"yes\">Chlamydomonas reinhardtii<\/jats:italic>\n                    . Loss of VIA1 causes hypersensitivity to photo-oxidative stress and rapid thylakoid swelling. VIA1 localizes to thylakoid membranes and directly binds Vesicle-Inducing Protein in Plastids 1 (VIPP1), an ESCRT-III-like protein essential for thylakoid biogenesis and remodeling. Structure-guided mutagenesis shows that this interaction is required for VIA1 function and is mediated by a winged-helix domain interface reminiscent of ESCRT-II\/ESCRT-III binding mode. VIA1 orthologs from cyanobacteria and land plants rescue the Chlamydomonas\n                    <jats:italic toggle=\"yes\">via1<\/jats:italic>\n                    mutant phenotype, and disruption of VIA1 in\n                    <jats:italic toggle=\"yes\">Synechocystis sp<\/jats:italic>\n                    . PCC 6803 impairs growth, especially under light stress. Together, these findings establish VIA1 as an evolutionarily conserved protein that contributes to thylakoid membrane homeostasis via its interaction with VIPP1.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2605816123","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:03:46Z","timestamp":1786993426000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["VIA1 is a conserved regulator of thylakoid membrane integrity that acts through VIPP1"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7498-4803","authenticated-orcid":false,"given":"Pamela","family":"Vetrano","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03anc3s24","id-type":"ROR","asserted-by":"publisher"}],"name":"Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter","place":["Vienna, Austria"]},{"id":[{"id":"https:\/\/ror.org\/05n3x4p02","id-type":"ROR","asserted-by":"publisher"}],"name":"Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey","family":"Krall","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03efmqc40","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Molecular Sciences, Arizona State University","place":["Tempe"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5211-2498","authenticated-orcid":false,"given":"Laura","family":"Martinez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03anc3s24","id-type":"ROR","asserted-by":"publisher"}],"name":"Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9019-0378","authenticated-orcid":false,"given":"Eleonora","family":"Traverso","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00240q980","id-type":"ROR","asserted-by":"publisher"}],"name":"Dipartimento di Biologia, Universit\u00e0 di Padova","place":["Padova, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0803-7591","authenticated-orcid":false,"given":"Tomas","family":"Morosinotto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00240q980","id-type":"ROR","asserted-by":"publisher"}],"name":"Dipartimento di Biologia, Universit\u00e0 di Padova","place":["Padova, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A. T.","family":"Irwin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03anc3s24","id-type":"ROR","asserted-by":"publisher"}],"name":"Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5072-0928","authenticated-orcid":false,"given":"Yuval","family":"Mazor","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03efmqc40","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Molecular Sciences, Arizona State University","place":["Tempe"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2703-1398","authenticated-orcid":false,"given":"Silvia","family":"Ramundo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03anc3s24","id-type":"ROR","asserted-by":"publisher"}],"name":"Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter","place":["Vienna, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-90-481-2863-1"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2015.02.025"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.01.007"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061500998"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.049841"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507287103"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.107.051714"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.113118"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1916946117"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1916948117"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061501198"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2007.01.005"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err357"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.111.092692"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.103606"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.00145"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.00532"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.05.011"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.05.041"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.05.042"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809467105"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165322"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.49577"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.116425"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","unstructured":"I. Yilmazer A conserved ESCRT-II-like protein participates in the biogenesis and maintenance of thylakoid membranes. bioRxiv [Preprint] (2023). https:\/\/doi.org\/10.1101\/2023.10.10.561251 (Accessed 10 October 2025).","DOI":"10.1101\/2023.10.10.561251"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-4-10"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.233734"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.201900429"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm259"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac278"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0370-6"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/148.4.1821"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.116.033035"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-08-0736"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad335"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","unstructured":"J. Hallgren DeepTMHMM predicts alpha and beta transmembrane proteins using deep neural networks. bioRxiv [Preprint] (2022). https:\/\/doi.org\/10.1101\/2022.04.08.487609 (Accessed 10 April 2022).","DOI":"10.1101\/2022.04.08.487609"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/364412a0"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.07.008"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.09.003"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","unstructured":"D. \u00c1lvarez-Salmoral AlphaBridge: Tools for the analysis of predicted biomolecular complexes. bioRxiv [Preprint] (2024) https:\/\/doi.org\/10.1101\/2024.10.23.619601 (Accessed 9 December 2025).","DOI":"10.1101\/2024.10.23.619601"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btad629"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.103051"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2012.12.020"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.408"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02914"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.061143"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3988"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki524"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.05.008"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3943"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1038"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2605816123","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2605816123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:04:14Z","timestamp":1786993454000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2605816123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":53,"journal-issue":{"issue":"34","published-print":{"date-parts":[[2026,8,25]]}},"alternative-id":["10.1073\/pnas.2605816123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2605816123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]},"assertion":[{"value":"2026-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2605816123"}}