{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:44:26Z","timestamp":1792176266511,"version":"4.4.0"},"reference-count":48,"publisher":"National Academy of Sciences","issue":"42","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"MEXT | JSPS KAKENHI Grant-in-Aid for Specially Promoted Research","award":["23H05405"],"award-info":[{"award-number":["23H05405"]}]},{"name":"MEXT | JSPS Grant-in-Aid for Scientific Research","award":["24K01627"],"award-info":[{"award-number":["24K01627"]}]},{"name":"MEXT | JSPS Grant-i-Aid for Scientific Research","award":["23K04960"],"award-info":[{"award-number":["23K04960"]}]},{"name":"MEXT | JSPS Grant-i-Aid for Scientific Research","award":["25K08856"],"award-info":[{"award-number":["25K08856"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:p>\n                    Labile zinc ions (Zn\n                    <jats:sup>2+<\/jats:sup>\n                    ) are stored in glutamatergic vesicles at specific excitatory synapses in the central nervous system and released into synaptic clefts in an activity-dependent manner. However, the precise roles of synaptic Zn\n                    <jats:sup>2+<\/jats:sup>\n                    remain unclear due to a lack of tools capable of analyzing Zn\n                    <jats:sup>2+<\/jats:sup>\n                    with synapse-level spatial resolution in vivo. Here, we developed neuroreceptor-based semisynthetic biosensors that function as integrating dosimeters, recording synaptic Zn\n                    <jats:sup>2+<\/jats:sup>\n                    by covalent chemistry in the living mouse brain. Using a chemical knock-in strategy, we introduced activity-based Zn\n                    <jats:sup>2+<\/jats:sup>\n                    probes with distinct affinities into endogenous \u03b1-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid or \u03b3-aminobutyric acid type A receptors. These in-brain-constructed Zn\n                    <jats:sup>2+<\/jats:sup>\n                    sensors enabled region-specific mapping of Zn\n                    <jats:sup>2+<\/jats:sup>\n                    in synaptic clefts in the living mouse brain. Imaging-based analyses revealed differences in apparent Zn\n                    <jats:sup>2+<\/jats:sup>\n                    levels at excitatory and inhibitory synapses across hippocampal regions during kainate-induced seizures, providing insights into the physiological and pathological functions of synaptic Zn\n                    <jats:sup>2+<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.2606947123","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T19:03:09Z","timestamp":1791831789000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Imaging and recording of synaptic zinc in the brain by covalent chemistry\u2013based semisynthetic biosensors"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3640-2290","authenticated-orcid":false,"given":"Hao","family":"Zhu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kpeqv85","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University","place":["Kyoto, Japan"]},{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Synthetic Chemistry for Molecular Systems, SANKEN (The Institute of Scientific and Industrial Research), The University of Osaka","place":["Ibaraki, 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