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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:p>\n                    Neural crest cells, a multipotent vertebrate stem cell population, undergo a tightly regulated epithelial\u2013mesenchymal transition (EMT) to delaminate from the neural tube and initiate migration. Neural crest EMT is controlled by a transient pulse of\n                    <jats:italic toggle=\"yes\">Draxin<\/jats:italic>\n                    , which acts as a molecular rheostat of canonical Wnt signaling, and is then rapidly downregulated by unknown mechanisms. To address this, here we use in vivo reporter assays and live RNA imaging to show that\n                    <jats:italic toggle=\"yes\">Draxin<\/jats:italic>\n                    mRNA is rapidly degraded posttranscriptionally via its 3\u2032 untranslated region. We found that\n                    <jats:italic toggle=\"yes\">Draxin<\/jats:italic>\n                    transcripts are targeted to cytoplasmic processing bodies (P-bodies), which are membrane-less sites of RNA processing and decay. Disruption of P-bodies by CRISPR\/Cas9-mediated depletion of the RNA helicase DDX6 prevented posttranscriptional downregulation of\n                    <jats:italic toggle=\"yes\">Draxin<\/jats:italic>\n                    and impaired neural crest EMT in vivo. These findings parallel observations in human patients carrying\n                    <jats:italic toggle=\"yes\">DDX6<\/jats:italic>\n                    mutations, who exhibit defects in P-body assembly and craniofacial abnormalities associated with neural crest dysfunction. Together, our results identify P-bodies as regulators of\n                    <jats:italic toggle=\"yes\">Draxin<\/jats:italic>\n                    mRNA decay and reveal a spatially organized mechanism of RNA turnover that controls neural crest EMT during vertebrate development.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2608103123","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T19:03:09Z","timestamp":1791831789000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["P-body-mediated RNA decay controls neural crest EMT"],"prefix":"10.1073","volume":"123","author":[{"given":"Arvind Arul Nambi","family":"Rajan","sequence":"first","affiliation":[{"name":"Department of Cell and Tissue Biology, University of California San Francisco","place":["San Francisco"]},{"id":[{"id":"https:\/\/ror.org\/043mz5j54","id-type":"ROR","asserted-by":"publisher"}],"name":"Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco","place":["San Francisco"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Piacentino","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dxps055","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Biology and Biological Engineering, California Institute of Technology","place":["Pasadena"]},{"id":[{"id":"https:\/\/ror.org\/00za53h95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cell Biology, Johns Hopkins University School of Medicine","place":["Baltimore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4274-1862","authenticated-orcid":false,"given":"Marianne E.","family":"Bronner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dxps055","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Biology and Biological Engineering, California Institute of Technology","place":["Pasadena"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4316-0333","authenticated-orcid":false,"given":"Erica J.","family":"Hutchins","sequence":"additional","affiliation":[{"name":"Department of Cell and Tissue Biology, University of California San Francisco","place":["San Francisco"]},{"id":[{"id":"https:\/\/ror.org\/043mz5j54","id-type":"ROR","asserted-by":"publisher"}],"name":"Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco","place":["San Francisco"]},{"id":[{"id":"https:\/\/ror.org\/05dxps055","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Biology and Biological Engineering, California Institute of Technology","place":["Pasadena"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2024.102400"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.07.004"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.12.041"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38019"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.11.007"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2012.03.010"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0237-9"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.06.028"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.12.001"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.05.027"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23107"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2017.07.015"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13091-8"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2019.10.003"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40556"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2018.05.009"},{"key":"e_1_3_4_17_2","first-page":"2194","article-title":"Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by Dact1\/2","volume":"143","author":"Rabad\u00e1n M. 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