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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:p>The glycosaminoglycan hyaluronan (HA) is an essential and ubiquitous component of human tissues and biofluids. The only known covalent modification of HA entails the attachment of heavy chains (HC) from the inter-alpha-inhibitor (I\u03b1I) family of proteoglycans, forming stable complexes (HC\u00b7HA) that arise during inflammation. In some contexts, HC\u00b7HA is thought to contribute to pathology, whereas in others it may form part of a protective pathway. However, its exact roles are not fully understood. Here, we report that HC modifications can protect HA from fragmentation by reactive oxygen species (ROS) produced during the inflammatory cascade. Using solid-state nanopore molecular size analysis, we show that HA is highly resistant to degradation from exogenous ROS in vitro when in the context of HC\u00b7HA complexes, while the unmodified HA polymer is fragmented rapidly under the same conditions. Experiments performed with admixtures of HA and unbound antioxidant proteins\u2014including HC-bearing components\u2014demonstrate that covalent HC attachment to the polysaccharide is necessary for the protection. Finally, we find that HA with high-HC content from \u201cinflammatory\u201d equine synovial fluid has increased resilience to ROS damage compared to low-HC\u00b7HA from a healthy joint. Collectively, these results demonstrate that covalent HC modification is an effective biological strategy for preserving HA integrity against ROS fragmentation, including in inflammatory conditions.<\/jats:p>","DOI":"10.1073\/pnas.2611716123","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:02:56Z","timestamp":1790622176000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["A role for heavy chain-modification in protecting hyaluronan from free radical fragmentation during inflammation"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7900-5362","authenticated-orcid":false,"given":"Suruchi","family":"Poddar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0207ad724","id-type":"ROR","asserted-by":"publisher"}],"name":"Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest University 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