{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:26:13Z","timestamp":1789658773708,"version":"4.0.1"},"reference-count":58,"publisher":"National Academy of Sciences","issue":"38","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2021-NR060094; RS-2023-NR077104; RS-2025-25435480"],"award-info":[{"award-number":["RS-2021-NR060094; RS-2023-NR077104; RS-2025-25435480"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["RS-2025-25410994"],"award-info":[{"award-number":["RS-2025-25410994"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"},{"id":"https:\/\/ror.org\/00fdzyk40","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,9,22]]},"abstract":"<jats:p>\n                    Arginine vasopressin maintains water homeostasis by regulating epithelial water permeability through complex transcriptional mechanisms in kidney collecting duct cells. Although CTNNB1 (\u03b2-catenin) functions as a transcriptional coregulator in vasopressin-responsive gene transcription, its role remains poorly understood. To identify CTNNB1-dependent components mediating the vasopressin-responsive transcription, we profiled transcriptomic changes following Ctnnb1 knockdown in mouse kidney collecting duct cells using RNA sequencing (RNA-Seq). RNA-Seq and promoter enrichment analyses identified bromodomain-containing proteins (TRIM28, TRIM33, BRD4, CREBBP, and EP300) as components of a CTNNB1-dependent complex regulating RNA Polymerase II (Pol II) activity. Biochemical analyses revealed physical interactions between TRIM28, CTNNB1, Pol II, and CDK9. Functionally, Trim28 knockdown blunted vasopressin-induced expression of the\n                    <jats:italic toggle=\"yes\">Aqp2<\/jats:italic>\n                    gene. Quantitative genomic binding assays demonstrated that TRIM28 is required for robust genomic occupancy and stabilization of Pol II at the transcription start site of\n                    <jats:italic toggle=\"yes\">Aqp2<\/jats:italic>\n                    . Additionally, dynamic formation of phase-separated nuclear TRIM28 condensates in response to vasopressin suggests that TRIM28-associated machinery functions at specialized chromatin hubs. These findings reveal that TRIM28 facilitates Pol II recruitment, pause release, and elongation upon vasopressin stimulation. Our study establishes the CTNNB1\u2013TRIM28 machinery as a critical transcriptional scaffold that controls Pol II dynamics and chromatin structure, thereby driving osmotic water reabsorption and urine concentration.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2611995123","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:55:17Z","timestamp":1789494917000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["The CTNNB1\u2013TRIM28 complex governs hormone-induced RNA polymerase II dynamics in kidney epithelial cells"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1420-4491","authenticated-orcid":false,"given":"Hyo-Ju","family":"Jang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University","place":["Daegu, Korea"]},{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Brain Korea 21 FOUR Kyungpook National University Convergence Educational Program, Department of Biomedical Science, Kyungpook National University","place":["Daegu, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6329-1499","authenticated-orcid":false,"given":"Euijung","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University","place":["Daegu, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0519-5858","authenticated-orcid":false,"given":"Hyun Jun","family":"Jung","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, School of Medicine, Kyungpook National University","place":["Daegu, Korea"]},{"id":[{"id":"https:\/\/ror.org\/04qn0xg47","id-type":"ROR","asserted-by":"publisher"}],"name":"Bioinformatics Core, Biomedical Research Institute, Kyungpook National University Hospital","place":["Daegu, Korea"]},{"id":[{"id":"https:\/\/ror.org\/00za53h95","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Nephrology, Department of Medicine, Johns Hopkins University School of Medicine","place":["Baltimore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1561-6508","authenticated-orcid":false,"given":"Tae-Hwan","family":"Kwon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040c17130","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of 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