{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:51:10Z","timestamp":1791312670201,"version":"4.3.1"},"reference-count":64,"publisher":"National Academy of Sciences","issue":"41","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["223125\/Z\/21\/Z"],"award-info":[{"award-number":["223125\/Z\/21\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"UKRI | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M011151\/1"],"award-info":[{"award-number":["BB\/M011151\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UKRI | Medical Research Council","doi-asserted-by":"publisher","award":["MR\/X020827\/1"],"award-info":[{"award-number":["MR\/X020827\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","award":["NIHR202129"],"award-info":[{"award-number":["NIHR202129"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","award":["NIHR GOSH BRC"],"award-info":[{"award-number":["NIHR GOSH BRC"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000833","name":"Rosetrees Trust","doi-asserted-by":"publisher","award":["PGL22\/100091"],"award-info":[{"award-number":["PGL22\/100091"]}],"id":[{"id":"10.13039\/501100000833","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,13]]},"abstract":"<jats:p>Cilia are microtubule-based organelles essential for sensing, motility, and fluid transport. Motile cilia rely on inner and outer dynein arms, large multiprotein motor complexes, for force generation and the characteristic bending motion required for ciliary beating. Before being incorporated into cilia, these dynein arms are preassembled in the cytoplasm by dynein axonemal assembly factors (DNAAFs). Defects in this assembly pathway often cause the inherited genetic condition primary ciliary dyskinesia (PCD), in which the loss of dynein arms impairs ciliary motility. The molecular mechanisms governing the precise and timely assembly of dynein arms by DNAAFs remain elusive. Here, we investigated the role of dynein axonemal assembly factor 19 (DNAAF19), a DNAAF frequently mutated in PCD patients with combined inner and outer dynein arm defects. Using biochemical, structural, and proteomic approaches, we find that DNAAF19 is a monomeric protein that interacts directly with the heterohexamer of RUVBL1 and RUVBL2, a multifunctional cochaperone complex involved in ciliary dynein arm assembly. The recurrent PCD-causing variant p.His154Pro partially destabilizes the interaction of DNAAF19 with RUVBL1-RUVBL2. Moreover, we identify Deleted in Primary Ciliary Dyskinesia forming a cocomplex with RUVBL1\/2-DNAAF19 in vivo and in vitro. This indicates similarity to R2TP, the HSP90 cochaperone composed of RUVBL1\/2-RPAP3-PIH1D1 that assembles different molecular machineries in cells, and to the RUVBL1\/2\u2013SPAG1\u2013PIH1D2 complex associated with ciliary dynein arm assembly and PCD. Our results suggest that DNAAF19 acts as a DNAAF in concert with RUVBL1\/2 and its interacting proteins, providing insights into the molecular coordination of dynein arm assembly and cell biology of PCD.<\/jats:p>","DOI":"10.1073\/pnas.2612755123","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:04:38Z","timestamp":1791309878000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["DNAAF19\u2013RUVBL1\/2 complex recruits multiple adaptors to promote dynein arm assembly"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1752-7639","authenticated-orcid":false,"given":"Mafalda","family":"de Almeida Gomes","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02jx3x895","id-type":"ROR","asserted-by":"publisher"}],"name":"Genetics and Genomic Medicine Department, University College London, Great Ormond Street Institute of Child Health, University College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2516-615X","authenticated-orcid":false,"given":"Emma","family":"Wood","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"name":"Astbury Centre for Structural Molecular Biology and School of Molecular and Cellular Biology, Faculty of Health and Life Sciences, University of Leeds","place":["Leeds, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8428-720X","authenticated-orcid":false,"given":"Thomas","family":"Burgoyne","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02jx3x895","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Ophthalmology, University College London","place":["London, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/00j161312","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Paediatric Respiratory Medicine, Department of Paediatric Respiratory Medicine, Royal Brompton Hospital, Guy\u2019s and St Thomas\u2019 NHS Foundation Trust","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Klose","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03a1kwz48","id-type":"ROR","asserted-by":"publisher"}],"name":"Core Facility for Medical Proteomics, University of T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1373-9700","authenticated-orcid":false,"given":"Denisa","family":"Batrinu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02jx3x895","id-type":"ROR","asserted-by":"publisher"}],"name":"Genetics and Genomic Medicine Department, University College London, Great Ormond Street Institute of Child Health, University College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2693-689X","authenticated-orcid":false,"given":"Karsten","family":"Boldt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03a1kwz48","id-type":"ROR","asserted-by":"publisher"}],"name":"Core Facility for Medical Proteomics, University of T\u00fcbingen","place":["T\u00fcbingen, Germany"]},{"id":[{"id":"https:\/\/ror.org\/03a1kwz48","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Ophthalmic Research, Center for Ophthalmology, University of T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn","family":"Carrington","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"name":"Astbury Centre for Structural Molecular Biology and School of Molecular and Cellular Biology, Faculty of Health and Life Sciences, University of Leeds","place":["Leeds, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda","family":"Halfon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"name":"Astbury Centre for Structural Molecular Biology and School of Molecular and Cellular Biology, Faculty of Health and Life Sciences, University of Leeds","place":["Leeds, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"George","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"name":"Astbury Centre for Structural Molecular Biology and School of Molecular and Cellular Biology, Faculty of Health and Life Sciences, University of Leeds","place":["Leeds, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1834-2485","authenticated-orcid":false,"given":"G. Nasir","family":"Khan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"name":"Astbury Centre for Structural Molecular Biology and School of Molecular and Cellular Biology, Faculty of Health and Life Sciences, University of Leeds","place":["Leeds, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6302-8997","authenticated-orcid":false,"given":"Tina","family":"Beyer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03a1kwz48","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Ophthalmic Research, Center for Ophthalmology, University of T\u00fcbingen","place":["T\u00fcbingen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3163-6293","authenticated-orcid":false,"given":"Hannah M.","family":"Mitchison","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02jx3x895","id-type":"ROR","asserted-by":"publisher"}],"name":"Genetics and Genomic Medicine Department, University College London, Great Ormond Street Institute of Child Health, University College London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8546-6341","authenticated-orcid":false,"given":"Takashi","family":"Ochi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/024mrxd33","id-type":"ROR","asserted-by":"publisher"}],"name":"Astbury Centre for Structural Molecular Biology and School of Molecular and Cellular Biology, Faculty of Health and Life Sciences, University of Leeds","place":["Leeds, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2020.11.007"},{"key":"e_1_3_4_2_2","unstructured":"M. A. Zariwala K. A. Despotes S. D. Davis \u201cPrimary Ciliary Dyskinesia\u201d in GeneReviews\u00ae [Internet] M. P. Adam . Eds. (University of Washington Seattle WA 1993\u20132026) https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK1122\/."},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2017-0436OC"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2025.103660"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2025.103651"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-74021-8_11"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a028076","article-title":"Axoneme structure from motile cilia","volume":"9","author":"Ishikawa T.","year":"2016","unstructured":"T. Ishikawa, Axoneme structure from motile cilia. Cold Spring Harb. Perspect. Biol. 9, a028076 (2016).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a028100"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.260408"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.264247"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lpm.2023.104171"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2017.11.001"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2020.08.010"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14279"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04431-1"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.259512"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2707"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1715915115"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07471"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2013.07.025"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.38497"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.263886"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2277"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01769-2023"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2017-209999"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.616425"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08337-5"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9051139"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01773-0"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2520079"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.113713"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15-08-0578"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03942-1"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-026-69157-x"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","unstructured":"H. Mu\u00f1oz-Hern\u00e1ndez CCDC103-mediated assembly of the R2C complex links RUVBL1-RUVBL2 to Primary Ciliary Dyskinesia. bioRxiv [Preprint] (2025). https:\/\/doi.org\/10.1101\/2025.09.11.675549.","DOI":"10.1101\/2025.09.11.675549"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2022.167760"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adg7492"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaw1616"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202109014"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20100489"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01112-1"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.06.008"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106106108"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.09.001"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom10040605"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.63042"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2020.04.010"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2020.103947"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4792"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319011471"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00140"},{"key":"e_1_3_4_54_2","doi-asserted-by":"crossref","unstructured":"E. Wood T. Ochi Cryo-EM structure of human CCDC103\/DNAAF19 in complex with RUVBL1\/2. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57214. Deposited 4 March 2026.","DOI":"10.2210\/pdb29jr\/pdb"},{"key":"e_1_3_4_55_2","doi-asserted-by":"crossref","unstructured":"E. Wood T. Ochi Composite cryo-EM map of the human DNAAF19-RUVBL1\/2-DPCD complex. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57241. Deposited 4 March 2026.","DOI":"10.2210\/pdb29kn\/pdb"},{"key":"e_1_3_4_56_2","unstructured":"E. Wood T. Ochi Consensus map of human DNAAF19-RUVBL1\/2-DPCD. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57215. Deposited 17 March 2026."},{"key":"e_1_3_4_57_2","unstructured":"E. Wood T. Ochi Focused map of human DNAAF19-RUVBL1\/2 without the external DII domains. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57217. Deposited 17 March 2026."},{"key":"e_1_3_4_58_2","unstructured":"E. Wood T. Ochi Focused map of the external DII domain of RUVBL2 in complex with DPCD. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57219. Deposited 17 March 2026."},{"key":"e_1_3_4_59_2","unstructured":"E. Wood T. Ochi Focused map of the external DII domain of RUVBL2 in complex with DPCD. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57221. Deposited 17 March 2026."},{"key":"e_1_3_4_60_2","unstructured":"E. Wood T. Ochi Focused map of the external DII domain of RUVBL2 in complex with DPCD. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57234. Deposited 17 March 2026."},{"key":"e_1_3_4_61_2","unstructured":"E. Wood T. Ochi Focused map of the external DII domain of RUVBL2 in complex with DPCD. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57238. Deposited 17 March 2026."},{"key":"e_1_3_4_62_2","unstructured":"E. Wood T. Ochi Focused map of the external DII domain of RUVBL2 in complex with DPCD. Electron Microscopy Database. https:\/\/www.ebi.ac.uk\/emdb\/EMD-57239. Deposited 17 March 2026."},{"key":"e_1_3_4_63_2","doi-asserted-by":"crossref","unstructured":"E. Wood T. Ochi Cryo-EM structure of human CCDC103\/DNAAF19 in complex with RUVBL1\/2. Protein Data Bank. https:\/\/www.rcsb.org\/structure\/29JR. Deposited 4 March 2026.","DOI":"10.2210\/pdb29jr\/pdb"},{"key":"e_1_3_4_64_2","doi-asserted-by":"crossref","unstructured":"E. Wood T. Ochi Composite cryo-EM map of the human DNAAF19-RUVBL1\/2-DPCD complex. Protein Data Bank. https:\/\/www.rcsb.org\/structure\/29KN. Deposited 4 March 2026.","DOI":"10.2210\/pdb29kn\/pdb"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2612755123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:04:51Z","timestamp":1791309891000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2612755123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":64,"journal-issue":{"issue":"41","published-print":{"date-parts":[[2026,10,13]]}},"alternative-id":["10.1073\/pnas.2612755123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2612755123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"assertion":[{"value":"2026-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2612755123"}}