{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:04:40Z","timestamp":1790701480238,"version":"4.1.0"},"reference-count":57,"publisher":"National Academy of Sciences","issue":"40","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["1"],"award-info":[{"award-number":["1"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["2"],"award-info":[{"award-number":["2"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:p>\n                    Insect herbivory triggers rapid local and systemic signaling in plants, including membrane depolarization, cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    elevation, and activation of jasmonate-mediated defense pathways. These early events are shaped by calcium-permeable ion channels and regulatory components that generate stimulus-specific Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signatures. The glutamate receptor-like channel-centric model of long-distance Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling is insufficient to explain the robustness of systemic responses to diverse damage and herbivore cues, indicating the involvement of additional Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels. Here, we identify CYCLIC NUCLEOTIDE GATED CHANNEL13 (CNGC13) as a plasma membrane\u2013localized, vasculature-expressed Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channel that is essential for systemic jasmonate signaling and defense against lepidopteran insect pest,\n                    <jats:italic toggle=\"yes\">Spodoptera litura<\/jats:italic>\n                    in\n                    <jats:italic toggle=\"yes\">Arabidopsis thaliana<\/jats:italic>\n                    .\n                    <jats:italic toggle=\"yes\">CNGC13<\/jats:italic>\n                    is rapidly induced by wounding and herbivory and is required for efficient propagation of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals from wounded to distal leaves. Loss of CNGC13 compromises the thioglucosidase (Ricca factor)-dependent breakdown of aliphatic glucosinolates into aglucones\/isothiocyanates within the vascular tissue, which are essential for wound-induced systemic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    wave propagation. Consequently,\n                    <jats:italic toggle=\"yes\">cngc13<\/jats:italic>\n                    mutants display reduced wound induced jasmonate accumulation in systemic leaves, reduced glucosinolate levels and enhanced herbivore susceptibility. CNGC13 is also required for\n                    <jats:italic toggle=\"yes\">At<\/jats:italic>\n                    Pep-induced Ca\n                    <jats:sup>2+<\/jats:sup>\n                    elevation and reactive oxygen species signaling and physically interacts with PLANT ELICITOR PEPTIDE RECEPTOR 2 (PEPR2) kinase domain. Consistently,\n                    <jats:italic toggle=\"yes\">pepr2<\/jats:italic>\n                    mutants display similar defects in glucosinolate aglucone production and systemic jasmonate accumulation. Together, our findings identify CNGC13 as a vasculature-localized Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -permeable channel that integrates multiple damage-cues, including Ricca factor and\n                    <jats:italic toggle=\"yes\">At<\/jats:italic>\n                    Pep-PEPR signaling, to drive and sustain systemic signaling and immune activation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2612869123","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:18:26Z","timestamp":1790698706000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["The Ca\n                    <sup>2+<\/sup>\n                    channel CYCLIC NUCLEOTIDE GATED CHANNEL13 regulates vasculature-mediated systemic Ca\n                    <sup>2+<\/sup>\n                    and jasmonate signaling on herbivory"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4384-1013","authenticated-orcid":false,"given":"Misha","family":"Kumari","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04zw11527","id-type":"ROR","asserted-by":"publisher"}],"name":"Biotechnology Research and Innovation Council-National Institute of Plant Genome Research","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramgopal","family":"Prajapati","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04zw11527","id-type":"ROR","asserted-by":"publisher"}],"name":"Biotechnology Research and Innovation Council-National Institute of Plant Genome Research","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahendra","family":"Pawar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04zw11527","id-type":"ROR","asserted-by":"publisher"}],"name":"Biotechnology Research and Innovation Council-National Institute of Plant Genome Research","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7263-1510","authenticated-orcid":false,"given":"Bhawana","family":"Sharma","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04zw11527","id-type":"ROR","asserted-by":"publisher"}],"name":"Biotechnology Research and Innovation Council-National Institute of Plant Genome Research","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Maria","family":"Orlando Marchesano","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00wjc7c48","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biosciences, University of Milan","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8516-4036","authenticated-orcid":false,"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04ew43640","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Plant Trait Design, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3139-7701","authenticated-orcid":false,"given":"Yong-Fei","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04ew43640","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Plant Trait Design, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2628-1176","authenticated-orcid":false,"given":"Alex","family":"Costa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00wjc7c48","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biosciences, University of Milan","place":["Milan, Italy"]},{"name":"Institute of Biophysics, National Research Council of Italy","place":["Milan, 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vectors for highly efficient CRISPR\/Cas9-mediated gene knockout in Arabidopsis thaliana","volume":"58","author":"Tsutsui H.","year":"2017","unstructured":"H. Tsutsui, T. Higashiyama, pKAMA-ITACHI vectors for highly efficient CRISPR\/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 58, 46\u201356 (2017).","journal-title":"Plant Cell Physiol."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2612869123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:19:02Z","timestamp":1790698742000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2612869123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":57,"journal-issue":{"issue":"40","published-print":{"date-parts":[[2026,10,6]]}},"alternative-id":["10.1073\/pnas.2612869123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2612869123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"2026-04-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2612869123"}}