{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T11:30:33Z","timestamp":1793532633495,"version":"4.5.0"},"reference-count":69,"publisher":"National Academy of Sciences","issue":"39","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012226","name":"MOE | Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["22hytd05"],"award-info":[{"award-number":["22hytd05"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"High-Level Young Talents Program (Provincial Matching Funds), Guangdong province","award":["2023HYSPT0708"],"award-info":[{"award-number":["2023HYSPT0708"]}]},{"name":"Major Project of Guangzhou National Laboratory","award":["GZNL2024A01017"],"award-info":[{"award-number":["GZNL2024A01017"]}],"id":[{"id":"https:\/\/ror.org\/03ybmxt82","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:p>\n                    The cGAS (cyclic GMP-AMP synthase)-STING (Stimulator of interferon genes) pathway plays a central role in antiviral innate immunity in vertebrates. Recent studies have shown that invertebrates possess a related cGLR (cGAS-like receptor)-STING pathway. However, due to the lack of direct evidence demonstrating viral evasion of cGLR-STING-mediated immunity, the functional importance of cGLR-STING pathway in antiviral responses remains uncertain in invertebrates. Here, we show that an insect picorna-like virus CrPV (Cricket Paralysis virus) inhibits STING-mediated antiviral activity by altering the transcription start site of\n                    <jats:italic toggle=\"yes\">STING<\/jats:italic>\n                    . Mechanistically, CrPV infection down-regulates several spliceosomal genes and disrupts pre-mRNA splicing. This splicing impairment is sufficient to stall RNA polymerase II in transcriptional initiation condensates, reduce global transcription efficiency, and reprogram the transcription start site of\n                    <jats:italic toggle=\"yes\">STING<\/jats:italic>\n                    . Such a shift diverts\n                    <jats:italic toggle=\"yes\">STING<\/jats:italic>\n                    expression from a functional antiviral isoform to a nonantiviral variant that partially suppresses STING activity, thereby disabling the cGLR-STING pathway. Our findings uncover an unconventional viral immune evasion strategy and an unexpected link between splicing suppression and STING-mediated antiviral response. Given the evolutionary conservation of splicing mechanisms and STING-like pathways, this mechanism may represent a broadly applicable regulatory principle in immune defense across species.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2613025123","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:25:03Z","timestamp":1790180703000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1,"title":["Splicing suppression alters the\n                    <i>STING<\/i>\n                    transcription start site to attenuate antiviral innate immunity"],"prefix":"10.1073","volume":"123","author":[{"given":"Jia","family":"Xu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0064kty71","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Biocontrol, School of Ecology, Sun Yat-Sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5929-7053","authenticated-orcid":false,"given":"Qiuchang","family":"Liao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0064kty71","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Biocontrol, School of Ecology, Sun Yat-Sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayin","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0064kty71","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Biocontrol, School of Ecology, Sun Yat-Sen University","place":["Shenzhen, 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Wu , clusterProfiler 4.0: A universal enrichment tool for interpreting omics data. Innovation 2, 100141 (2021).","journal-title":"Innovation"},{"key":"e_1_3_4_66_2","unstructured":"J. Xu Splicing suppression alters STING transcription start site to attenuate antiviral innate immunity [CrPV vs mi]. Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE345930. Deposited 2 September 2026."},{"key":"e_1_3_4_67_2","unstructured":"J. Xu Splicing suppression alters STING transcription start site to attenuate antiviral innate immunity [DCV FHV vs mi]. Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE345932. Deposited 2 September 2026."},{"key":"e_1_3_4_68_2","unstructured":"J. Xu Splicing suppression alters STING transcription start site to attenuate antiviral innate immunity [dsPrp8 vs dsGFP]. Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE345934. 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