{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:48:02Z","timestamp":1790430482326,"version":"4.1.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,12,18]]},"abstract":"<jats:p>\n                    To investigate the regulation of endocytosis by Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ,\n we have made capacitance measurements in the synaptic terminal of\n depolarizing bipolar cells from the retina of goldfish. After a\n brief depolarization, all of the excess membrane was retrieved rapidly\n (\u03c4 \u22481 s). But when the rise in free [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ] was\n reduced by the introduction of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    buffers\n [1,2-bis(2-aminophenoxy)ethane-\n                    <jats:italic>N,N,N<\/jats:italic>\n                    \u2032\n                    <jats:italic>,N<\/jats:italic>\n                    \u2032-tetraacetate\n (BAPTA) or EGTA], a large fraction of the membrane was retrieved by a\n second, slower mechanism (\u03c4 \u2265 10 s). The block of fast\n endocytosis by EGTA could be overcome by increasing the amplitude of\n the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    current, demonstrating that Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx was the trigger for fast endocytosis. These manipulations of the\n Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signal altered the relative proportions of fast and\n slow endocytosis but did not modulate the rate constants of these\n processes. A brief stimulus that triggered fast endocytosis did not\n generate a significant rise in the spatially averaged\n [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ], indicating that Ca\n                    <jats:sup>2+<\/jats:sup>\n                    regulated\n endocytosis through an action close to the active zone. The slow mode\n of retrieval occurred at the resting [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]. These\n results demonstrate that Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx couples fast\n endocytosis and exocytosis at this synapse.\n                  <\/jats:p>","DOI":"10.1073\/pnas.261311698","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:36:44Z","timestamp":1027679804000},"page":"15282-15287","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":81,"title":["Calcium influx selects the fast mode of endocytosis in the synaptic terminal of retinal bipolar cells"],"prefix":"10.1073","volume":"98","author":[{"given":"Guilherme","family":"Neves","sequence":"first","affiliation":[{"name":"Medical Research Council Laboratory of Molecular Biology,\r Hills Road, Cambridge CB2 2QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Gomis","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratory of Molecular Biology,\r Hills Road, Cambridge CB2 2QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon","family":"Lagnado","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratory of Molecular Biology,\r Hills Road, Cambridge CB2 2QH, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2001,12,4]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1967.sp008183"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80238-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-11-03844.1989"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.57.2.315"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.57.2.499"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(94)90025-6"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.21.12677"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(99)80046-4"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80236-6"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(97)01083-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/367735a0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.181ad.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00243-4"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.883"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.11.5567"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80208-1"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/29079"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/83949"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-21-08614.1998"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-03-00949.2000"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/370652a0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.571ba.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-15-06309.1999"},{"key":"e_1_3_2_24_2","unstructured":"Burrone J. 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