{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:59:01Z","timestamp":1790272741042,"version":"4.1.0"},"reference-count":84,"publisher":"National Academy of Sciences","issue":"39","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB-1942565"],"award-info":[{"award-number":["MCB-1942565"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB-2448119"],"award-info":[{"award-number":["MCB-2448119"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","award":["ALTF 177-2024"],"award-info":[{"award-number":["ALTF 177-2024"]}],"id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:p>\n                    RNA modifications play an important role in biological processes. Mapping the diversity of RNA chemistry and studying the biological function of individual modifications remains an outstanding challenge in many organisms. In particular, RNA modifications remain poorly studied across most bacterial systems. Our group previously developed RNA-mediated activity-based protein profiling (RNABPP), a reactivity-based strategy employing metabolic labeling and quantitative proteomics to profile RNA modification writer enzymes in human cells. Here, we adapt this approach to characterize RNA-modifying enzymes in bacteria. We apply metabolic labeling with 5-fluoropyrimidine nucleosides and phase separation-based enrichment of RNA-protein complexes (RNABPP-PS) to profile RNA pyrimidine modifying enzymes in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Bacillus subtilis<\/jats:italic>\n                    . We identify known and putative bacterial pyrimidine C5 methyltransferases, pseudouridine synthases, and dihydrouridine synthases, demonstrating the utility of our approach. Further, we find the carboxymethylaminomethyluridine (cnmn\n                    <jats:sup>5<\/jats:sup>\n                    U)-forming enzyme MnmG (GidA), supporting the existence of a covalent protein\u2013RNA intermediate during the catalytic cycle. Finally, we use RNABPP-PS in\n                    <jats:italic toggle=\"yes\">B. subtilis<\/jats:italic>\n                    to identify YfjO, an uncharacterized protein that is homologous to 5-methyluridine (m\n                    <jats:sup>5<\/jats:sup>\n                    U) methyltransferases. We use nucleoside and oligonucleotide mass spectrometry to establish that YfjO (which we rename as RlmS) installs m\n                    <jats:sup>5<\/jats:sup>\n                    U620 in the 23S rRNA (U576 in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    ), a modification specific to the\n                    <jats:italic toggle=\"yes\">B. subtilis<\/jats:italic>\n                    ribosome. We characterize\n                    <jats:italic toggle=\"yes\">\u0394yfjO B. subtilis<\/jats:italic>\n                    , which exhibits impaired growth and protein translation, concomitant with a defect in 70S ribosome assembly. Taken together, our study establishes a versatile platform for RNA modifying enzyme discovery and characterization in bacteria and illuminates species-specific rRNA modification chemistry in\n                    <jats:italic toggle=\"yes\">B. subtilis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.2613881123","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:59:47Z","timestamp":1790269187000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Activity-based profiling of bacterial RNA-modifying enzymes reveals species-specific 5-methyluridine modification in\n                    <i>Bacillus subtilis<\/i>\n                    23S rRNA"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6780-9494","authenticated-orcid":false,"given":"Qais Z.","family":"Jaber","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan J.","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9385-4424","authenticated-orcid":false,"given":"Isao","family":"Masuda","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00ysqcn41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Molecular Biology, Thomas Jefferson University","place":["Philadelphia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ming","family":"Hou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00ysqcn41","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Molecular Biology, Thomas Jefferson University","place":["Philadelphia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0508-9975","authenticated-orcid":false,"given":"Ralph E.","family":"Kleiner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Princeton University","place":["Princeton"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0319-0"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.05.045"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00622-x"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2016.1267096"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.12.007"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/167483a0"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2020.06.010"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1956510"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-01108-x"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01633-w"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2418-2"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1191"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-024-01726-x"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2401981121"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-021-00342-0"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2024.2442856"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1506749112"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae222"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf1531"},{"key":"e_1_3_4_20_2","volume-title":"Quantitative RNA Pseudouridine Landscape Reveals Dynamic Modification Patterns and Evolutionary Conservation across Bacterial Species","author":"Xu L.","year":"2026","unstructured":"L. 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