{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:06:44Z","timestamp":1791274004300,"version":"4.1.0"},"reference-count":31,"publisher":"National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,12,18]]},"abstract":"<jats:p>\n                    Type IV pili (Tfp) of\n                    <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                    , the\n Gram-negative etiologic agent of gonorrhea, facilitate colonization of\n the human host. Tfp are assumed to play a key role in the initial\n adherence to human epithelial cells by virtue of the associated adhesin\n protein PilC. To examine the structural and functional basis for\n adherence in more detail, we identified potential genes encoding\n polypeptides sharing structural similarities to PilE (the Tfp subunit)\n within the\n                    <jats:italic>N. gonorrhoeae<\/jats:italic>\n                    genome sequence database. We\n show here that a fiber subunit-like protein, termed PilV, is essential\n to organelle-associated adherence but dispensable for Tfp biogenesis\n and other pilus-related phenotypes, including autoagglutination,\n competence for natural transformation, and twitching motility. The\n adherence defect in\n                    <jats:italic>pilV<\/jats:italic>\n                    mutants cannot be attributed to\n reduced levels of piliation, defects in fiber anchoring to the\n bacterial cell surface, or to unstable pilus expression related to\n organelle retraction. PilV is expressed at low levels relative to PilE\n and copurifies with Tfp fibers in a PilC-dependent fashion. Purified\n Tfp from\n                    <jats:italic>pilV<\/jats:italic>\n                    mutants contain PilC adhesin at reduced\n levels. Taken together, these data support a model in which PilV\n functions in adherence by promoting the functional display of PilC in\n the context of the pilus fiber.\n                  <\/jats:p>","DOI":"10.1073\/pnas.261574998","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:36:44Z","timestamp":1027679804000},"page":"15276-15281","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":127,"title":["<i>Neisseria gonorrhoeae<\/i>\n                    PilV, a type IV pilus-associated protein essential to human epithelial cell adherence"],"prefix":"10.1073","volume":"98","author":[{"given":"Hanne C.","family":"Winther-Larsen","sequence":"first","affiliation":[{"name":"Biotechnology Center of Oslo and Department of Microbiology,\r Institute of Pharmacy, University of Oslo, 0316 Oslo, Norway;\r Department of Microbiology and Molecular Genetics,\r Harvard Medical School, Boston, MA 02115; Microscopy\r Branch, Rocky Mountain Laboratories, National Institute of Allergy\r and Infectious Diseases, National Institutes of Health, Hamilton, MT\r 59840; and Department of Infectious Diseases and\r Immunology, Utrecht University, NL-3584 CL Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Finn Terje","family":"Hegge","sequence":"additional","affiliation":[{"name":"Biotechnology Center of Oslo and Department of Microbiology,\r Institute of Pharmacy, University of Oslo, 0316 Oslo, Norway;\r Department of Microbiology and Molecular Genetics,\r Harvard Medical School, Boston, MA 02115; Microscopy\r Branch, Rocky Mountain Laboratories, National Institute of Allergy\r and Infectious Diseases, National Institutes of Health, Hamilton, MT\r 59840; and Department of Infectious Diseases and\r Immunology, Utrecht University, NL-3584 CL Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Wolfgang","sequence":"additional","affiliation":[{"name":"Biotechnology Center of Oslo and Department of Microbiology,\r Institute of Pharmacy, University of Oslo, 0316 Oslo, Norway;\r Department of Microbiology and Molecular Genetics,\r Harvard Medical School, Boston, MA 02115; Microscopy\r Branch, Rocky Mountain Laboratories, National Institute of Allergy\r and Infectious Diseases, National Institutes of Health, Hamilton, MT\r 59840; and Department of Infectious Diseases and\r Immunology, Utrecht University, NL-3584 CL Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley F.","family":"Hayes","sequence":"additional","affiliation":[{"name":"Biotechnology Center of Oslo and Department of Microbiology,\r Institute of Pharmacy, University of Oslo, 0316 Oslo, Norway;\r Department of Microbiology and Molecular Genetics,\r Harvard Medical School, Boston, MA 02115; Microscopy\r Branch, Rocky Mountain Laboratories, National Institute of Allergy\r and Infectious Diseases, National Institutes of Health, Hamilton, MT\r 59840; and Department of Infectious Diseases and\r Immunology, Utrecht University, NL-3584 CL Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos P. 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