{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:57:13Z","timestamp":1792173433644,"version":"4.4.0"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"42","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["03407-2023 RGPIN"],"award-info":[{"award-number":["03407-2023 RGPIN"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["044661-2018 RGPIN"],"award-info":[{"award-number":["044661-2018 RGPIN"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:p>\n                    Suberin deposition in specialized cells is a critical aspect of plant protection, providing both water retention and pathogen protection functions. Tissue specific suberization during plant development is mirrored by its wound induction in cells that do not normally suberize and provides a robust experimental system to investigate the process. Suberized cells are characterized by a cell wall\u2013associated lignin-like poly(phenolic) domain, and an ester-linked poly(aliphatic) domain, with the latter often laid down in distinct lamellar bands between the cell wall and plasma membrane. Previous work provides evidence that poly(phenolic) deposition precedes, and is necessary for, poly(aliphatic) deposition. To test this further, we generated RNAi knockdown lines of three wound-induced peroxidase genes,\n                    <jats:italic toggle=\"yes\">PRX19, PRX55,<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">PRX105<\/jats:italic>\n                    , in potato (\n                    <jats:italic toggle=\"yes\">Solanum tuberosum<\/jats:italic>\n                    ), the tubers of which produce an abundance of suberin upon wounding. Peroxidases catalyze the oxidative free radical coupling of phenolic monomers to form the suberin poly(phenolic) domain. The wound-induced suberin of PRX19-RNAi tubers had severely reduced poly(phenolics), reduced poly(aliphatics), and no apparent lamella. Functionally, wound-induced PRX19-RNAi tubers were more permeable to water loss and susceptible to bacterial soft rot. Overall, our data support the hypothesis that poly(phenolic) domain assembly is a strict requirement for subsequent deposition of aliphatic lamella within wound-induced suberin.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2616035123","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:39:04Z","timestamp":1792172344000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Poly(phenolic) deposition is a prerequisite to poly(aliphatic) deposition for functional potato suberin"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6441-2823","authenticated-orcid":false,"given":"Mina","family":"Esfandiari","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02grkyz14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, University of Western Ontario","place":["London, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idunnuayo","family":"Alabi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02grkyz14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, University of Western Ontario","place":["London, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2582-5503","authenticated-orcid":false,"given":"Sangeeta","family":"Dhaubhadel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02grkyz14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, University of Western Ontario","place":["London, Canada"]},{"id":[{"id":"https:\/\/ror.org\/02e9w7q14","id-type":"ROR","asserted-by":"publisher"}],"name":"London Research and Development Centre, Agriculture and Agri-Food Canada","place":["London, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1209-3200","authenticated-orcid":false,"given":"Mark A.","family":"Bernards","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02grkyz14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, University of Western Ontario","place":["London, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17765"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1056008"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0167"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2010.06.004"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2015.00062"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants11040555"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.13.7382"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf9020834"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-021-00862-9"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12374-025-09471-x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.121.1.135"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.81.2.487"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2012728117"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2004.06.023"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02464885"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0031-9317.2004.00301.x"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.11.070"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0110248"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2022.113529"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants14101433"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6619"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.091090"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2017.12.011"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2020.115428"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.114.124776"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2025.03.009"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-009-0587-z"},{"key":"e_1_3_4_28_2","first-page":"59","article-title":"Wound-induced metabolism in potato (Solanum tuberosum) tubers: Biosynthesis of aliphatic domain monomers","volume":"2006","author":"Yang W.-L.","year":"2007","unstructured":"W.-L. Yang, M. A. Bernards, Wound-induced metabolism in potato (Solanum tuberosum) tubers: Biosynthesis of aliphatic domain monomers. Plant Signal Behav. 2006, 59\u201366 (2007).","journal-title":"Plant Signal Behav."},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","unstructured":"A. Charkowski K. Sharma M. L. Parker G. A. Secor J. Elphinstone \u201cBacterial Diseases of Potato\u201d in The Potato Crop H. Campos O. Ortiz Eds. (Springer Cham 2020) pp. 351\u2013388.","DOI":"10.1007\/978-3-030-28683-5_10"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.208.4447.990"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2023.1017388"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-004-1364-9"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(96)00500-6"},{"key":"e_1_3_4_34_2","first-page":"193","article-title":"GATEWAYTM vectors for Agrobacterium-mediated plant transformation","volume":"7","author":"Karimi M.","year":"2002","unstructured":"M. Karimi, D. Inz\u00e9, A. Depicker, GATEWAYTM vectors for Agrobacterium-mediated plant transformation. TIPS 7, 193\u2013195 (2002).","journal-title":"TIPS"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants13212995"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11540-012-9212-y"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/s42269-023-01056-3"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(98)80052-6"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eri285"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04682.x"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2021.112885"},{"key":"e_1_3_4_42_2","first-page":"16","volume-title":"Electron Microscopy 2nd Edition","author":"Bozzola J. J.","year":"1999","unstructured":"J. J. Bozzola, L. D. Russell, \u201cSpecimen preparation for transmission electron microscopy\u201d in Electron Microscopy 2nd Edition (Principles and techniques for biologists Jones and Bartlett, Sudbury MA, 1999), pp. 16\u201347."},{"key":"e_1_3_4_43_2","volume-title":"The Role of Peroxidase in Suberin Assembly in Potato","author":"Esfandiari M.","year":"2026","unstructured":"M. Esfandiari, The Role of Peroxidase in Suberin Assembly in Potato (Western University, London, ON, Canada, 2026)."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2616035123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:40:00Z","timestamp":1792172400000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2616035123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,16]]},"references-count":43,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2026,10,20]]}},"alternative-id":["10.1073\/pnas.2616035123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2616035123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,16]]},"assertion":[{"value":"2026-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2616035123"}}