{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T17:41:33Z","timestamp":1792777293321,"version":"4.5.0"},"reference-count":46,"publisher":"National Academy of Sciences","issue":"43","license":[{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Science Foundation of China","award":["22372061 22572061"],"award-info":[{"award-number":["22372061 22572061"]}]},{"name":"Guangdong S&T Program","award":["2024B1111130002"],"award-info":[{"award-number":["2024B1111130002"]}]},{"name":"Guangzhou Science and Technology Plan Project","award":["2024A03J0163"],"award-info":[{"award-number":["2024A03J0163"]}]},{"name":"Major Project of Guangzhou National Laboratory (","award":["GZNL2024A03001"],"award-info":[{"award-number":["GZNL2024A03001"]}],"id":[{"id":"https:\/\/ror.org\/03ybmxt82","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital & Shenzhen Hospital","award":["E010224011"],"award-info":[{"award-number":["E010224011"]}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,27]]},"abstract":"<jats:p>\n                    Single-cell RNA sequencing (scRNA-seq) is constrained by rapid RNA degradation during sample storage, necessitating cold-chain logistics. Here we present spatially confined biosilicification (SCB), wherein RNA is encapsulated within a nanoscopic layer of amorphous silicon dioxide, as a highly effective methodology enabling ambient-temperature preservation of single-cell transcriptomes. By judiciously incorporating a silicification inhibitor, SCB achieves spatiotemporal control over both intra- and extracellular silica deposition, preventing excessive crosslinking and cell aggregation while preserving RNA biointegrity and accessibility. scRNA-seq performed with biosilicified cells (Si-scRNA-seq) maintained high transcriptomic fidelity with fresh samples (R\n                    <jats:sup>2<\/jats:sup>\n                    = 0.95 after 7 d) extending RNA half-life to 54.8 d at 25 \u00b0C, a 685-fold improvement compared to unprotected samples. Furthermore, Si-scRNA-seq confers robust resistance against oxidative and UV degradation. Compatible with mainstream platforms at ~0.1 RMB per sample, Si-scRNA-seq successfully resolves cellular heterogeneity in clinical lung, liver, and ovarian cancer specimens after extended room-temperature storage. Si-scRNA-seq provides a scalable and cold-chain\u2013free solution for single-cell transcriptomics in research and clinical settings.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2617730123","type":"journal-article","created":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T17:32:01Z","timestamp":1792776721000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Si-scRNA-seq: Enabling ambient-stable single-cell transcriptomics through spatially confined biosilicification"],"prefix":"10.1073","volume":"123","author":[{"given":"Liang","family":"Zhou","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0530pts50","id-type":"ROR","asserted-by":"publisher"}],"name":"Guangdong Engineering Research Center of Low-Carbon Synthetic Biotechnology, State Key Laboratory of Pulp and Paper Engineering, School of Biology and Biological Engineering, South China University of Technology","place":["Guangzhou, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Ruan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0530pts50","id-type":"ROR","asserted-by":"publisher"}],"name":"Guangdong Engineering Research Center of Low-Carbon Synthetic Biotechnology, State Key Laboratory of Pulp and Paper Engineering, School of Biology and Biological Engineering, South China University of Technology","place":["Guangzhou, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"He","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02drdmm93","id-type":"ROR","asserted-by":"publisher"}],"name":"National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College","place":["Shenzhen, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangting","family":"Xie","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ybmxt82","id-type":"ROR","asserted-by":"publisher"}],"name":"Guangzhou National Laboratory","place":["Guangzhou, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyu","family":"Qi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ybmxt82","id-type":"ROR","asserted-by":"publisher"}],"name":"Guangzhou National Laboratory","place":["Guangzhou, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2211-2823","authenticated-orcid":false,"given":"Xiaobao","family":"Cao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ybmxt82","id-type":"ROR","asserted-by":"publisher"}],"name":"Guangzhou National Laboratory","place":["Guangzhou, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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Zhou Si-scRNA-seq: Enabling ambient-stable single-cell transcriptomics through spatially confined biosilicification. CNGB Sequence Archive (CNSA). https:\/\/db.cngb.org\/data_resources\/project\/CNP0008735. 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