{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:30:48Z","timestamp":1786113048015,"version":"build-2736575974"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"32","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001869","name":"Academia Sinica","doi-asserted-by":"publisher","award":["AS-IA-112-L01"],"award-info":[{"award-number":["AS-IA-112-L01"]}],"id":[{"id":"10.13039\/501100001869","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["MOST 109-2311-B-001-028-MY3"],"award-info":[{"award-number":["MOST 109-2311-B-001-028-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["MOST 109-2811-B-001-527 MOST-110-2811-B-001-573 NSTC 111-2811-B-001-068"],"award-info":[{"award-number":["MOST 109-2811-B-001-527 MOST-110-2811-B-001-573 NSTC 111-2811-B-001-068"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,8,11]]},"abstract":"<jats:p>\n                    The synchronization of the internal circadian clock with the external 24-h diel cycle is critical for plant growth and fitness. LIGHT-REGULATED WD proteins (LWDs) are essential for sustaining a precise and robust circadian clock in\n                    <jats:italic toggle=\"yes\">Arabidopsis<\/jats:italic>\n                    [Y. Wang\n                    <jats:italic toggle=\"yes\">et al.<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Plant Cell<\/jats:italic>\n                    <jats:bold>23<\/jats:bold>\n                    , 486\u2013498 (2011); J. F. Wu\n                    <jats:italic toggle=\"yes\">et al.<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">Nat Commun.<\/jats:italic>\n                    <jats:bold>7<\/jats:bold>\n                    , 13181 (2016)]. However, the action mechanisms by which LWDs orchestrate the expression of clock genes remain largely elusive. We systematically identified 219 LWD1-interacting protein partners at three Zeitgeber times (ZTs) using a TurboID-mediated proximity labeling approach. Selected interacting proteins were experimentally verified as new regulators of the\n                    <jats:italic toggle=\"yes\">Arabidopsis<\/jats:italic>\n                    circadian clock. Our data also support that LWD1-PSEUDO RESPONSE REGULATORS (PRRs)-TOPLESS (TPL) complex represses the morning gene\n                    <jats:italic toggle=\"yes\">CIRCADIAN CLOCK ASSOCIATED<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">CCA1<\/jats:italic>\n                    ), shaping the expression trough of\n                    <jats:italic toggle=\"yes\">CCA1<\/jats:italic>\n                    in the late afternoon\/early evening under diel cycles. Our results revealed a new corepressor role of LWD1, working in conjunction with epigenetic regulators and transcription factors to underpin the precise operation of the circadian clock.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2618203123","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T17:36:00Z","timestamp":1785951360000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Unveiling previously undescribed circadian clock regulators and action mechanisms via TurboID-based profiling of the LWD1 interactome in\n                    <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7325-1255","authenticated-orcid":false,"given":"Chun-Kai","family":"Huang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00jj3h083","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Plant and 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Huang Unveiling previously undescribed circadian clock regulators and action mechanisms via TurboID-based profiling of the LWD1 interactome in Arabidopsis thaliana. PRIDE Archive. https:\/\/www.ebi.ac.uk\/pride\/archive\/projects\/PXD062258. Deposited 21 July 2026."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2618203123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T17:36:26Z","timestamp":1785951386000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2618203123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,5]]},"references-count":51,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2026,8,11]]}},"alternative-id":["10.1073\/pnas.2618203123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2618203123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,5]]},"assertion":[{"value":"2026-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2618203123"}}