{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:08:08Z","timestamp":1790096888455,"version":"4.0.1"},"reference-count":86,"publisher":"National Academy of Sciences","issue":"39","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["OGB-190360"],"award-info":[{"award-number":["OGB-190360"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT-191897"],"award-info":[{"award-number":["PJT-191897"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN2015-04022"],"award-info":[{"award-number":["RGPIN2015-04022"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2021-03154"],"award-info":[{"award-number":["RGPIN-2021-03154"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:p>\n                    During development, many neurons prune their neurites. While many pruning events are activity-dependent, some neurons undergo stereotyped and developmentally regulated neurite pruning, and our understanding of the signaling pathways that mediate this form of pruning remains limited. In this study, using the PDB motor neuron in\n                    <jats:italic toggle=\"yes\">Caenorhabditis elegans<\/jats:italic>\n                    , we show that the Wnt-calcium signaling pathway is required for stereotyped neurite pruning during development. We found that mutants of\n                    <jats:italic toggle=\"yes\">itr-1\/<\/jats:italic>\n                    IP3 receptor and two calcium-dependent kinases,\n                    <jats:italic toggle=\"yes\">unc-43\/<\/jats:italic>\n                    CaMKII and\n                    <jats:italic toggle=\"yes\">pkc-2\/<\/jats:italic>\n                    PKC, exhibit neurite pruning defects. Genetic analysis suggested that they function downstream of\n                    <jats:italic toggle=\"yes\">lin-44\/<\/jats:italic>\n                    Wnt in neurite pruning. Human CaMKIIA can induce neurite pruning in\n                    <jats:italic toggle=\"yes\">C. elegans<\/jats:italic>\n                    , and mutations in CaMKII genes in patients with intellectual disabilities affect its pruning function. In vivo calcium imaging revealed that PDB neurites exhibit calcium transients during neurite pruning, which are regulated at least in part by\n                    <jats:italic toggle=\"yes\">lin-44<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">itr-1<\/jats:italic>\n                    . Furthermore, we demonstrate that\n                    <jats:italic toggle=\"yes\">pkc-2<\/jats:italic>\n                    regulates neurite pruning through clathrin-mediated endocytosis. Together, our work reveals the critical functions of Wnt\u2013calcium signaling in neurite pruning.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2619924123","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:12:31Z","timestamp":1790093551000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["LIN-44\/Wnt controls developmental neurite pruning via UNC-43\/CaMKII and PKC-2\/PKC in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7770-6317","authenticated-orcid":false,"given":"Menghao","family":"Lu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Graduate Program in Cell and Developmental Biology, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Life Sciences Institute, The University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey S. H.","family":"Lin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Life Sciences Institute, The University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuki","family":"Kurashina","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Graduate Program in Cell and Developmental Biology, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Life Sciences Institute, The University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8091-4483","authenticated-orcid":false,"given":"Kota","family":"Mizumoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Zoology, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Graduate Program in Cell and Developmental Biology, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Life Sciences Institute, The University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Djavad Mowafaghian Centre for Brain Health, The University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.28.061604.135632"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013038"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-014-1729-6"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1787"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05337-6"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8511592"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.11.026"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2023.01.032"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507393102"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.07.050"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202312043"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00267-8"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2254"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.07.057"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2347"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50583"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.31.060407.125649"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.180116"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.145"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.8463"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200303107"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/37138"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200211094"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80310-8"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/35011068"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.17.12701"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/417295a"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.181305"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0183-09.2009"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20846"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.09.023"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1986.0056"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/46072"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/156.3.1069"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.119.302063"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.32451"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.23647"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(00)02028-X"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3229"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.134.4.885"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/375073a0"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.20.6066"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/143.3.1219"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01616"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/351751a0"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.11.009"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-08-0422"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5464-09.2010"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80945-9"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0435-6"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08478-6"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.04.007"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5261-13.2014"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1016-8478(23)13192-X"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.1.60"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(00)00125-3"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1261"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2654-2"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2741"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2017.10.003"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13772"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-018-0288-4"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2023.12.016"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-025-03316-4"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2019.04.001"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.240861"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-025-03607-w"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.12.2337"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)01142-X"},{"key":"e_1_3_4_70_2","first-page":"4279","article-title":"Regulation of dendrite growth and maintenance by exocytosis","volume":"128","author":"Peng Y.","year":"2015","unstructured":"Y. Peng , Regulation of dendrite growth and maintenance by exocytosis. J. Cell Sci. 128, 4279\u20134292 (2015).","journal-title":"J. Cell Sci."},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1111\/dgd.12218"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.707486"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-12-0871"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22227"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-18-07879.2002"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2023.08.002"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/77.1.71"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04550.x"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb04966.x"},{"key":"e_1_3_4_80_2","article-title":"A rapid protocol for integrating extrachromosomal arrays with high transmission rate into the C. elegans genome","volume":"10","author":"Mariol M. C.","year":"2013","unstructured":"M. C. Mariol, L. Walter, S. Bellemin, K. Gieseler, A rapid protocol for integrating extrachromosomal arrays with high transmission rate into the C. elegans genome. J. Vis. Exp. 10, e50773 (2013).","journal-title":"J. Vis. Exp."},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkad041"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.120.303564"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.102028"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyae214"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.118.200778"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12868-017-0391-y"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2619924123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:13:33Z","timestamp":1790093613000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2619924123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":86,"journal-issue":{"issue":"39","published-print":{"date-parts":[[2026,9,29]]}},"alternative-id":["10.1073\/pnas.2619924123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2619924123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,22]]},"assertion":[{"value":"2026-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2619924123"}}