{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:58:01Z","timestamp":1791392281444,"version":"4.3.3"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"41","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K14577"],"award-info":[{"award-number":["JP22K14577"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K01309"],"award-info":[{"award-number":["JP24K01309"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP26H01626"],"award-info":[{"award-number":["JP26H01626"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JPMXP1323015482"],"award-info":[{"award-number":["JPMXP1323015482"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,13]]},"abstract":"<jats:p>\n                    Classical cadherin-mediated cell\u2013cell adhesion is important for maintaining the animal body and defects in adhesion cause severe diseases. The combination of\n                    <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                    - (between cells) and\n                    <jats:italic toggle=\"yes\">cis<\/jats:italic>\n                    - (on the same membrane) interactions of the extracellular domains of cadherins is necessary for cadherin clustering in cell\u2013cell junctions, which is essential for robust tissue formation. However, the solution structure and interaction dynamics of\n                    <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                    \/\n                    <jats:italic toggle=\"yes\">cis<\/jats:italic>\n                    -binding cadherin complexes have not been observed because of technical limitations; therefore, the cadherin clustering mechanism is not well understood. In this study, we reconstituted\n                    <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                    \/\n                    <jats:italic toggle=\"yes\">cis<\/jats:italic>\n                    -binding cadherin complexes on membranes and visualized their structure and dynamics using high-speed atomic force microscopy. Our method revealed\n                    <jats:italic toggle=\"yes\">trans<\/jats:italic>\n                    -strand-swap interactions through flipping and hopping motions and\n                    <jats:italic toggle=\"yes\">cis<\/jats:italic>\n                    -interactions, which contributed to multiple interaction patterns on the membrane. In addition, we observed cadherin complex structures that were distinct from those in crystal structures. These results suggest that cadherins adopt dynamic and diverse conformations through multiple assembly pathways on the membrane.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2620441123","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:35:51Z","timestamp":1791390951000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["<i>Trans<\/i>\n                    - and\n                    <i>cis<\/i>\n                    -interaction dynamics of cadherin complexes on membranes visualized by high-speed atomic force microscopy"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0378-6937","authenticated-orcid":false,"given":"Shigetaka","family":"Nishiguchi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01703db54","id-type":"ROR","asserted-by":"publisher"}],"name":"Molecular Biosystems Research Institute, National Institute of Advanced Industrial Science and Technology","place":["Ikeda, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4568-9043","authenticated-orcid":false,"given":"Tadaomi","family":"Furuta","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05dqf9946","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Life Science and Technology, Institute of Science Tokyo","place":["Yokohama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0263-5312","authenticated-orcid":false,"given":"Takayuki","family":"Uchihashi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04chrp450","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Physics, Nagoya University","place":["Nagoya, Japan"]},{"id":[{"id":"https:\/\/ror.org\/055n47h92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Creative Research, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences","place":["Okazaki, Japan"]},{"id":[{"id":"https:\/\/ror.org\/04chrp450","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Glyco-core Research, Nagoya University","place":["Nagoya, Japan"]},{"id":[{"id":"https:\/\/ror.org\/04chrp450","id-type":"ROR","asserted-by":"publisher"}],"name":"Quantum-Based Frontier Research Hub for Industry Development, Nagoya University","place":["Nagoya, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/329341a0"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2012.03.004"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2927"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3136"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2022.11.004"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a003129"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394311-8.00016-9"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071559"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1784"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1604012113"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201006113"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2010.11.016"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpclett.9b01500"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10183"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2019845118"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.12.003"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.211400898"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-030722-113353"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2208067119"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00666a005"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc8003155"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2002.2774"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e07-01-0084"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086957"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2051"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1314303110"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/374327a0"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2053230X15003878"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2004563117"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2019.02.008"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.04.010"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2018.06.003"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62097"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2017.04.001"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2457"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.12703\/r\/12-25"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a028761"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.03.062"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208349109"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2104090118"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1063\/5.0111017"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja907649w"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2302047120"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.115365"},{"key":"e_1_3_4_45_2","unstructured":"S. Nishiguchi T. Furuta T. Uchihashi Trans- and cis-interaction dynamics of cadherin complexes on membranes visualized by high-speed atomic force microscopy. Mendeley Data. https:\/\/data.mendeley.com\/datasets\/8wz7mgzyxb\/2. Deposited 25 August 2026."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.2620441123","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:36:32Z","timestamp":1791390992000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/10.1073\/pnas.2620441123"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,7]]},"references-count":45,"journal-issue":{"issue":"41","published-print":{"date-parts":[[2026,10,13]]}},"alternative-id":["10.1073\/pnas.2620441123"],"URL":"https:\/\/doi.org\/10.1073\/pnas.2620441123","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,7]]},"assertion":[{"value":"2026-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2620441123"}}