{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:59:03Z","timestamp":1791392343421,"version":"4.3.3"},"reference-count":78,"publisher":"National Academy of Sciences","issue":"41","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI172958"],"award-info":[{"award-number":["R01AI172958"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"HHS | NIH | National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["T32DC012280"],"award-info":[{"award-number":["T32DC012280"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["T32AI007405"],"award-info":[{"award-number":["T32AI007405"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,13]]},"abstract":"<jats:p>\n                    Clinical data link the prevalent respiratory tract anaerobe\n                    <jats:italic toggle=\"yes\">Prevotella<\/jats:italic>\n                    with reduced pneumonia mortality, but the mechanisms directing\n                    <jats:italic toggle=\"yes\">Prevotella<\/jats:italic>\n                    regulation of lung immune homeostasis are unclear. Here, single-cell RNA sequencing was employed to define the transcriptional immune signatures underlying improved clearance of\n                    <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n                    following lung exposure to\n                    <jats:italic toggle=\"yes\">Prevotella melaninogenica<\/jats:italic>\n                    . Overall, we observed a substantial shift in myeloid cell transcriptional programming from interferon-dominant to a more antibacterial profile in\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    \u2013infected mice after pre-exposure to\n                    <jats:italic toggle=\"yes\">P. melaninogenica<\/jats:italic>\n                    , correlating with increased macrophage and neutrophil phagocytosis of\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    and improved pathogen clearance. In neutrophils, tumor necrosis factor (TNF) signaling through TNFR2 was essential for increased antimicrobial function. Moreover, improved defense required CCR2-dependent monocyte-derived macrophages, with selective enrichment of more a mature Cxcl3+ population which was distinct from the hallmark\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    \u2013associated C1qa+ population enriched in the absence of effective clearance. Together, these findings inform the myeloid cell transcriptional changes associated with natural infection resistance mediated by pulmonary microbial exposures.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2623525123","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:35:51Z","timestamp":1791390951000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Myeloid cell reprogramming drives enhanced defense against\n                    <i>Streptococcus pneumoniae<\/i>\n                    lung infection following exposure to commensal\n                    <i>Prevotella<\/i>"],"prefix":"10.1073","volume":"123","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9350-9441","authenticated-orcid":false,"given":"Sara N.","family":"Stoner","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03wmf1y16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of 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N. Stoner Data from \u201cMyeloid cell reprogramming drives enhanced defense against Streptococcus pneumoniae lung infection following exposure to commensal Prevotella.\u201d Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE313404. 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