{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:57:03Z","timestamp":1792173423728,"version":"4.4.0"},"reference-count":56,"publisher":"National Academy of Sciences","issue":"42","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI156096"],"award-info":[{"award-number":["AI156096"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:p>\n                    Calcium ion (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) transfer from other organelles is central to mitochondrial metabolism and cell survival, yet how mechanical forces regulate interorganelle communication remains unclear. Here we report a lysosome-to-mitochondrion pathway in\n                    <jats:italic toggle=\"yes\">Trypanosoma cruzi<\/jats:italic>\n                    , the etiologic agent of Chagas disease, mediated by the mechano\/osmosensitive channel TcPiezo2. Using ultrastructural, biochemical, and live-cell superresolution imaging, we define lysosome\u2013mitochondrion contact sites (LMCs) that enable direct Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transfer. TcPiezo2 triggers Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release, which generates perilysosomal nanodomains that drives mitochondrial Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake via the voltage-dependent anion channel and the mitochondrial Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uniporter complex. Disruption of TcPiezo2-dependent Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transfer impairs mitochondrial metabolism, perturbs redox homeostasis, induces autophagy, and eventually leads to parasite death. These findings establish LMCs as a signaling platform that directly regulates interorganelle Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transfer and mitochondrial bioenergetics.\n                  <\/jats:p>","DOI":"10.1073\/pnas.2627551123","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:39:04Z","timestamp":1792172344000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":0,"title":["Membrane contact activation of Piezo2 drives lysosome\u2013mitochondrion Ca\n                    <sup>2+<\/sup>\n                    transfer and signaling in\n                    <i>Trypanosoma cruzi<\/i>"],"prefix":"10.1073","volume":"123","author":[{"given":"Guozhong","family":"Huang","sequence":"first","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia","place":["Athens, Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4229-8784","authenticated-orcid":false,"given":"Roberto","family":"Docampo","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia","place":["Athens, Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)01787-7"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2003.09.001"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0070"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.4.1535"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00277-3"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2020.102284"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.749655"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20091051"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1097"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","DOI":"10.1128\/mmbr.00042-23","article-title":"Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes","volume":"88","author":"Docampo R.","year":"2023","unstructured":"R. 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