{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:11:58Z","timestamp":1788405118079,"version":"build-2803163510"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,3,4]]},"abstract":"<jats:p>\n                    Fast cholinergic neurotransmission between superior cervical ganglion neurons (SCGNs) in cell culture is initiated by N-type Ca\n                    <jats:sup>2+<\/jats:sup>\n                    currents through Ca\n                    <jats:sub>v<\/jats:sub>\n                    2.2 channels. To test the ability of different Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -channel subtypes to initiate synaptic transmission in these cells, SCGNs were injected with cDNAs encoding Ca\n                    <jats:sub>v<\/jats:sub>\n                    1.2 channels, which conduct L-type currents, Ca\n                    <jats:sub>v<\/jats:sub>\n                    2.1 channels, which conduct P\/Q-type Ca\n                    <jats:sup>2+<\/jats:sup>\n                    currents, and Ca\n                    <jats:sub>v<\/jats:sub>\n                    2.3 channels, which conduct R-type Ca\n                    <jats:sup>2+<\/jats:sup>\n                    currents. Exogenously expressed Ca\n                    <jats:sub>v<\/jats:sub>\n                    2.1 channels were localized in nerve terminals, as assessed by immunocytochemistry with subtype-specific antibodies, and these channels effectively initiated synaptic transmission. Injection with cDNA encoding Ca\n                    <jats:sub>v<\/jats:sub>\n                    2.3 channels yielded a lower level of presynaptic labeling and synaptic transmission, whereas injection with cDNA encoding Ca\n                    <jats:sub>v<\/jats:sub>\n                    1.2 channels resulted in no presynaptic labeling and no synaptic transmission. Our results show that exogenously expressed Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels can mediate synaptic transmission in SCGNs and that the specificity of reconstitution of neurotransmission (Ca\n                    <jats:sub>v<\/jats:sub>\n                    2.1 &gt; Ca\n                    <jats:sub>v<\/jats:sub>\n                    2.3 \u226b Ca\n                    <jats:sub>v<\/jats:sub>\n                    1.2) follows the same order as in neurons\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The specificity of reconstitution of neurotransmission parallels the specificity of trafficking of these Ca\n                    <jats:sub>v<\/jats:sub>\n                    channels to nerve terminals.\n                  <\/jats:p>","DOI":"10.1073\/pnas.262787299","type":"journal-article","created":{"date-parts":[[2003,3,4]],"date-time":"2003-03-04T13:36:00Z","timestamp":1046784960000},"page":"2813-2818","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":58,"title":["Subtype-selective reconstitution of synaptic transmission in sympathetic ganglion neurons by expression of exogenous calcium channels"],"prefix":"10.1073","volume":"100","author":[{"given":"Sumiko","family":"Mochida","sequence":"first","affiliation":[{"name":"Department of Physiology, Tokyo Medical University, Tokyo 160-8402, Japan; and Department of Pharmacology, University of Washington, Seattle, WA 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