{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:02:56Z","timestamp":1791424976145,"version":"4.3.3"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1970,11]]},"abstract":"<jats:p>\n                    \u03b1-Bungarotoxin, a polypeptide of mol wt 8000 purified from the venom of\n                    <jats:italic>Bungarus multicinctus<\/jats:italic>\n                    , blocks irreversibly and specifically the excitation by cholinergic agonists on the isolated electroplax and on purified membrane fragments\n                    <jats:italic>in vitro<\/jats:italic>\n                    . The toxin also blocks the\n                    <jats:italic>in vitro<\/jats:italic>\n                    binding of decamethonium to a protein recently isolated from electric tissue. This observation strengthens our earlier conclusion that this protein is the cholinergic receptor macromolecule.\n                  <\/jats:p>","DOI":"10.1073\/pnas.67.3.1241","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:23:48Z","timestamp":1149042228000},"page":"1241-1247","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":462,"title":["Use of a Snake Venom Toxin to Characterize the Cholinergic Receptor Protein"],"prefix":"10.1073","volume":"67","author":[{"given":"Jean-Pierre","family":"Changeux","sequence":"first","affiliation":[{"name":"DEPARTMENT DE BIOLOGIE MOL\u00c9CULAIRE, INSTITUT PASTEUR, PARIS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiki","family":"Kasai","sequence":"additional","affiliation":[{"name":"DEPARTMENT DE BIOLOGIE MOL\u00c9CULAIRE, INSTITUT PASTEUR, PARIS"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Yuan","family":"Lee","sequence":"additional","affiliation":[{"name":"PHARMACOLOGICAL INSTITUTE, COLLEGE OF MEDICINE, NATIONAL TAIWAN UNIVERSITY, TAIPEI, CHINA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1970,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.67.3.1241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:38:54Z","timestamp":1649846334000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.67.3.1241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,11]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1970,11]]}},"alternative-id":["10.1073\/pnas.67.3.1241"],"URL":"https:\/\/doi.org\/10.1073\/pnas.67.3.1241","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,11]]},"assertion":[{"value":"1970-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}