{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:45:15Z","timestamp":1781109915337,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1970,11]]},"abstract":"<jats:p>\n                    The biotin carboxylase component of acetyl CoA carboxylase has been purified approximately 2000 times from\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . This protein, which catalyzes the carboxylation of free\n                    <jats:italic>d<\/jats:italic>\n                    -biotin, is free of the biotin-containing carboxyl carrier protein, is homogeneous by polyacrylamide gel electrophoresis and analytical ultracentrifugation, and has been crystallized. Biotin carboxylase, with a molecular weight of approximately 100,000, is composed of two 50,000-dalton subunits. The catalytic capacity of biotin carboxylase is markedly enhanced by ethanol (11 times at 15% v\/v), and certain other organic solvents; this may mimic an effector-mediated response. The kinetic effect is exclusively on the maximal velocity of the reaction. Activation by ethanol is reversible and not accompanied by aggregation or disaggregation of the enzyme.\n                  <\/jats:p>","DOI":"10.1073\/pnas.67.3.1353","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:23:48Z","timestamp":1149042228000},"page":"1353-1360","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":54,"title":["Enzymatic Carboxylation of Biotin: Molecular and Catalytic Properties of a Component Enzyme of Acetyl CoA Carboxylase"],"prefix":"10.1073","volume":"67","author":[{"given":"Peter","family":"Dimroth","sequence":"first","affiliation":[{"name":"DEPARTMENT OF PHYSIOLOGICAL CHEMISTRY, THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE, BALTIMORE, MARYLAND 21205"},{"name":"DEPARTMENT OF BIOCHEMISTRY, NEW YORK UNIVERSITY SCHOOL OF MEDICINE, NEW YORK CITY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ras B.","family":"Guchhait","sequence":"additional","affiliation":[{"name":"DEPARTMENT OF PHYSIOLOGICAL CHEMISTRY, THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE, BALTIMORE, MARYLAND 21205"},{"name":"DEPARTMENT OF BIOCHEMISTRY, NEW YORK UNIVERSITY SCHOOL OF MEDICINE, NEW YORK CITY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Stoll","sequence":"additional","affiliation":[{"name":"DEPARTMENT OF PHYSIOLOGICAL CHEMISTRY, THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE, BALTIMORE, MARYLAND 21205"},{"name":"DEPARTMENT OF BIOCHEMISTRY, NEW YORK UNIVERSITY SCHOOL OF MEDICINE, NEW YORK CITY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Daniel","family":"Lane","sequence":"additional","affiliation":[{"name":"DEPARTMENT OF PHYSIOLOGICAL CHEMISTRY, THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE, BALTIMORE, MARYLAND 21205"},{"name":"DEPARTMENT OF BIOCHEMISTRY, NEW YORK UNIVERSITY SCHOOL OF MEDICINE, NEW YORK CITY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1970,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.67.3.1353","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:38:43Z","timestamp":1649846323000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.67.3.1353"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,11]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1970,11]]}},"alternative-id":["10.1073\/pnas.67.3.1353"],"URL":"https:\/\/doi.org\/10.1073\/pnas.67.3.1353","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,11]]},"assertion":[{"value":"1970-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}