{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:52:32Z","timestamp":1781103152953,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1971,1]]},"abstract":"<jats:p>\n                    A vitamin D\n                    <jats:sub>3<\/jats:sub>\n                    metabolite in intestine more polar than 25-hydroxy-vitamin D\n                    <jats:sub>3<\/jats:sub>\n                    (25-OH D\n                    <jats:sub>3<\/jats:sub>\n                    ) has been detected by countercurrent distribution. The intestinal metabolite is found also after administration of labeled hydroxy D\n                    <jats:sub>3<\/jats:sub>\n                    , indicating that it arises from vitamin D\n                    <jats:sub>3<\/jats:sub>\n                    via the intermediate 25-hydroxy derivative. The more polar metabolite is localized in the nuclear-chromatin fraction and appears in the gut before the physiological response to vitamin D\n                    <jats:sub>3<\/jats:sub>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    3.5 \u03bcg of the intestinal metabolite was isolated from 1250 chickens; the resulting purified material proved to be a potent mediator of calcium absorption. On a weight basis, it was at least 5 times as effective as vitamin D\n                    <jats:sub>3<\/jats:sub>\n                    , and acted 3 times faster than either hydroxy D\n                    <jats:sub>3<\/jats:sub>\n                    or D\n                    <jats:sub>3<\/jats:sub>\n                    in stimulating intestinal calcium transport in the rachitic chick. It is proposed that this as yet uncharacterized steroid represents the active form of vitamin D\n                    <jats:sub>3<\/jats:sub>\n                    in the intestine.\n                  <\/jats:p>","DOI":"10.1073\/pnas.68.1.177","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:24:15Z","timestamp":1149042255000},"page":"177-181","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":101,"title":["A Rapidly Acting Metabolite of Vitamin D\n                    <sub>3<\/sub>"],"prefix":"10.1073","volume":"68","author":[{"given":"M. R.","family":"Haussler","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia (19104)"},{"name":"USDA, National Animal Disease Laboratory, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. W.","family":"Boyce","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia (19104)"},{"name":"USDA, National Animal Disease Laboratory, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. T.","family":"Littledike","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia (19104)"},{"name":"USDA, National Animal Disease Laboratory, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, University of Pennsylvania, Philadelphia (19104)"},{"name":"USDA, National Animal Disease Laboratory, Ames, Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1971,1]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.68.1.177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:40:21Z","timestamp":1649846421000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.68.1.177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1971,1]]}},"alternative-id":["10.1073\/pnas.68.1.177"],"URL":"https:\/\/doi.org\/10.1073\/pnas.68.1.177","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,1]]},"assertion":[{"value":"1971-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}