{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:43:01Z","timestamp":1791078181079,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1971,10]]},"abstract":"<jats:p>\n                    Biotinyl-\n                    <jats:italic>p<\/jats:italic>\n                    -nitrophenyl ester (BNP), an active-ester derivative of biotin, irreversibly inactivates biotin transport in the yeast\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    . Transport inactivation is progressive with time and occurs at concentrations of the ester as low as 10\n                    <jats:sup>-7<\/jats:sup>\n                    M. In the presence of sodium azide, a reagent known to block biotin accumulation in yeast, the derivative is still effective. The specificity of inactivation by the ester is revealed by the following findings: (\n                    <jats:italic>a<\/jats:italic>\n                    ) Biotinyl-\n                    <jats:italic>p<\/jats:italic>\n                    -nitroanilide and acetyl-\n                    <jats:italic>p<\/jats:italic>\n                    -nitrophenyl ester do not affect biotin transport; (\n                    <jats:italic>b<\/jats:italic>\n                    ) the nitrophenyl ester does not affect the transport of lysine and aspartic acid, or that of L-sorbose; (\n                    <jats:italic>c<\/jats:italic>\n                    ) inactivation of biotin transport by the ester is partially prevented when the cells are incubated with it in the presence of relatively high concentrations of biotin.\n                  <\/jats:p>","DOI":"10.1073\/pnas.68.10.2604","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:25:16Z","timestamp":1149042316000},"page":"2604-2607","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":48,"title":["Irreversible Inhibition of Biotin Transport in Yeast by Biotinyl-\n                    <i>p<\/i>\n                    -nitrophenyl Ester"],"prefix":"10.1073","volume":"68","author":[{"given":"Jeffrey M.","family":"Becker","sequence":"first","affiliation":[{"name":"Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meir","family":"Wilchek","sequence":"additional","affiliation":[{"name":"Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ephraim","family":"Katchalski","sequence":"additional","affiliation":[{"name":"Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1971,10]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.68.10.2604","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:47:43Z","timestamp":1649846863000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.68.10.2604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1971,10]]}},"alternative-id":["10.1073\/pnas.68.10.2604"],"URL":"https:\/\/doi.org\/10.1073\/pnas.68.10.2604","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,10]]},"assertion":[{"value":"1971-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}