{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:39:18Z","timestamp":1781105958433,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1972,10]]},"abstract":"<jats:p>\n                    Yeast cells contain an RNA polymerase factor, \u03c0, which is a heat-stable protein with an apparent molecular weight of 12,000. This factor stimulates transcription of calf-thymus, salmon-sperm, yeast-nuclear, and T4-phage DNA. It stimulates transcription by each of the four yeast-nuclear RNA polymerases, by rat-liver RNA polymerases I and II, and by\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    RNA polymerase. \u03c0-Factor can cause each of the eukaryotic RNA polymerases to become insensitive to rifamycin AF-013, but does not stop inhibition of\n                    <jats:italic>E. coli<\/jats:italic>\n                    RNA polymerase by rifamycin AF-013. Stimulation of transcription by \u03c0-factor is general, and does not apply only to a limited class of genes. Apparently, \u03c0-factor stimulates transcription by increasing the proportion of RNA polymerase binding events that leads to the initiation of RNA chains.\n                  <\/jats:p>","DOI":"10.1073\/pnas.69.10.2818","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:32:40Z","timestamp":1149042760000},"page":"2818-2822","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["Transcription in Yeast: A Factor that Stimulates Yeast RNA Polymerases"],"prefix":"10.1073","volume":"69","author":[{"given":"Ernesto Di","family":"Mauro","sequence":"first","affiliation":[{"name":"Department of Genetics, University of Washington, Seattle, Wash. 98195"},{"name":"Department of Biochemistry, University of Washington, Seattle, Wash. 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelis P.","family":"Hollenberg","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Washington, Seattle, Wash. 98195"},{"name":"Department of Biochemistry, University of Washington, Seattle, Wash. 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin D.","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Washington, Seattle, Wash. 98195"},{"name":"Department of Biochemistry, University of Washington, Seattle, Wash. 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1972,10]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.69.10.2818","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:42:56Z","timestamp":1649846576000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.69.10.2818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1972,10]]}},"alternative-id":["10.1073\/pnas.69.10.2818"],"URL":"https:\/\/doi.org\/10.1073\/pnas.69.10.2818","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,10]]},"assertion":[{"value":"1972-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}