{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:52:23Z","timestamp":1781110343232,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1972,12]]},"abstract":"<jats:p>\n                    Tetrodotoxin from Japanese puffer fish has been labeled with tritium and purified from the crude mixture obtained. The interaction between the purified [\n                    <jats:sup>3<\/jats:sup>\n                    H]tetrodotoxin and membrane suspensions from the olfactory nerve of long-nosed garfish has been investigated by equilibrium dialysis. Tetrodotoxin binds to membrane suspensions with a dissociation constant\n                    <jats:italic>\n                      K\n                      <jats:sub>D<\/jats:sub>\n                    <\/jats:italic>\n                    = 8.3 nM. The nerve preparation binds 42 pmol of [\n                    <jats:sup>3<\/jats:sup>\n                    H]tetrodotoxin\/g of wet tissue at saturating toxin concentrations. With various hydrolytic enzymes, the binding component is shown to be a protein embedded in a phospholipid environment. The binding is inhibited below pH 4.0 and is not stable towards heat. Tetrodotoxin binding is not inhibited by the local anesthetic, procaine.\n                  <\/jats:p>","DOI":"10.1073\/pnas.69.12.3634","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:33:27Z","timestamp":1149042807000},"page":"3634-3637","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":44,"title":["Partial Characterization of a Tetrodotoxin-Binding Component from Nerve Membrane"],"prefix":"10.1073","volume":"69","author":[{"given":"Theodore I.","family":"Benzer","sequence":"first","affiliation":[{"name":"Church Laboratory of Chemical Biology, Division of Chemistry of Chemical Engineering, California Institute of Technology, Pasadena, Calif. 91109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Raftery","sequence":"additional","affiliation":[{"name":"Church Laboratory of Chemical Biology, Division of Chemistry of Chemical Engineering, California Institute of Technology, Pasadena, Calif. 91109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1972,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.69.12.3634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:23:36Z","timestamp":1649845416000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.69.12.3634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1972,12]]}},"alternative-id":["10.1073\/pnas.69.12.3634"],"URL":"https:\/\/doi.org\/10.1073\/pnas.69.12.3634","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,12]]},"assertion":[{"value":"1972-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}