{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:16:14Z","timestamp":1781108174973,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1972,2]]},"abstract":"<jats:p>\n                    Membrane microfilaments are found throughout the animal world in situations suggesting that they fulfill a critical role in providing normal cell shape and plasticity. We have hypothesized that hereditary spherocytosis, a congenital hemolytic anemia associated with intrinsically rigid and mishapen erythrocytes, might result from genetically defective microfilaments in erythrocyte membranes. By using three different drugs (vinblastine, colchicine, and strychnine) that share one common attribute\u2014that of potently precipitating purified microfilamentous protein\u2014we have provided support for this hypothesis. Thus, all the known\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    characteristics of hereditary spherocytes are reproduced in normal erythrocytes briefly exposed to these precipitating agents.\n                  <\/jats:p>","DOI":"10.1073\/pnas.69.2.471","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:32:44Z","timestamp":1149042764000},"page":"471-474","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":63,"title":["Membrane Microfilaments of Erythrocytes: Alteration in Intact Cells Reproduces the Hereditary Spherocytosis Syndrome"],"prefix":"10.1073","volume":"69","author":[{"given":"Harry","family":"Jacob","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Minnesota Medical School, Minneapolis, Minn. 55455"},{"name":"Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Mann. 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Amsden","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota Medical School, Minneapolis, Minn. 55455"},{"name":"Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Mann. 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"White","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota Medical School, Minneapolis, Minn. 55455"},{"name":"Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Mann. 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1972,2]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.69.2.471","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:28:56Z","timestamp":1649845736000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.69.2.471"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1972,2]]}},"alternative-id":["10.1073\/pnas.69.2.471"],"URL":"https:\/\/doi.org\/10.1073\/pnas.69.2.471","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,2]]},"assertion":[{"value":"1972-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}