{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:03:13Z","timestamp":1791896593167,"version":"4.3.4"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1972,3]]},"abstract":"<jats:p>\n                    The reduction of nitrogen, acetylene, azide, and cyanide at various oxygen concentrations by nitrogenase from\n                    <jats:italic>Azotobacter vinelandii<\/jats:italic>\n                    was measured with a well-defined system. Oxygen inhibited the reduction of each substrate uncompetitively. The inhibition constants (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    ) were 0.014, 0.023, 0.008, and 0.003 atm of oxygen for reduction of nitrogen, acetylene, azide, and cyanide, respectively. The system used included ATP-generating components, subcellular particles from\n                    <jats:italic>A. vinelandii<\/jats:italic>\n                    with high nitrogenase specific activity, and illuminated spinach chloroplasts plus carriers to supply electrons. Oxygen did not affect the photochemical electron donating system, but it did inhibit nitrogenase-dependent ATP hydrolysis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.69.3.672","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:33:44Z","timestamp":1149042824000},"page":"672-675","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":39,"title":["Nature of Oxygen Inhibition of Nitrogenase from\n                    <i>Azotobacter vinelandii<\/i>"],"prefix":"10.1073","volume":"69","author":[{"given":"Peter P.","family":"Wong","sequence":"first","affiliation":[{"name":"The Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wis. 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. H.","family":"Burris","sequence":"additional","affiliation":[{"name":"The Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wis. 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1972,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.69.3.672","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:37:41Z","timestamp":1649846261000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.69.3.672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1972,3]]}},"alternative-id":["10.1073\/pnas.69.3.672"],"URL":"https:\/\/doi.org\/10.1073\/pnas.69.3.672","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,3]]},"assertion":[{"value":"1972-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}