{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:32:18Z","timestamp":1781105538457,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1972,4]]},"abstract":"<jats:p>\n                    Stimulation of catecholamine release was studied in the isolated adrenal of the cat during retrograde perfusion. Theophylline, adenosine 3\u2032:5\u2032-cyclic monophosphate (cyclic AMP), and dibutyryl-cyclic AMP stimulated catecholamine release in adrenal chromaffin tissue; adenosine, 2\u2032-AMP, 3\u2032-AMP, and 5\u2032-AMP were ineffective. Addition of theophylline for 5-15 min had no effect on catecholamine release induced by KCl or nicotine, but it significantly increased the responses to cyclic AMP and its dibutyryl derivative. Glands perfused with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free Locke's solution for 30-180 min rapidly lost their responsiveness to KCl or nicotine. In contrast, exposure to Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free medium for 180 min had no effect on secretory responses to either the cyclic nucleotides, themselves, or to methylxanthine-induced potentiation of cyclic AMP responses. Thus, dibutyryl-cyclic AMP, cyclic AMP, and theophylline do not require extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    to release adrenal catecholamines. They may act by translocating intracellular bound Ca\n                    <jats:sup>2+<\/jats:sup>\n                    or by a mechanism independent of calcium.\n                  <\/jats:p>","DOI":"10.1073\/pnas.69.4.834","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:35:07Z","timestamp":1149042907000},"page":"834-836","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":68,"title":["Stimulation of Release of Adrenal Catecholamine by Adenosine 3\u2032:5\u2032-Cyclic Monophosphate and Theophylline in the Absence of Extracellular Ca\n                    <sup>2+<\/sup>"],"prefix":"10.1073","volume":"69","author":[{"given":"Michael J.","family":"Peach","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Virginia, School of Medicine, Charlottesville, Va. 22903"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1972,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.69.4.834","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:41:42Z","timestamp":1649846502000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.69.4.834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1972,4]]}},"alternative-id":["10.1073\/pnas.69.4.834"],"URL":"https:\/\/doi.org\/10.1073\/pnas.69.4.834","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,4]]},"assertion":[{"value":"1972-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}