{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:09:11Z","timestamp":1793297351610,"version":"4.5.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,11]]},"abstract":"<jats:p>\n                    The construction of new plasmid DNA species by\n                    <jats:italic>in vitro<\/jats:italic>\n                    joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules. Functional plasmids can be obtained by reassociation of endonuclease-generated fragments of larger replicons, as well as by joining of plasmid DNA molecules of entirely different origins.\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.11.3240","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:37:55Z","timestamp":1149043075000},"page":"3240-3244","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":1382,"title":["Construction of Biologically Functional Bacterial Plasmids\n                    <i>In Vitro<\/i>"],"prefix":"10.1073","volume":"70","author":[{"given":"Stanley N.","family":"Cohen","sequence":"first","affiliation":[{"name":"Department of Medicine, Stanford University School of Medicine, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie C. Y.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stanford University School of Medicine, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert W.","family":"Boyer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of California of San Francisco, San Francisco, Calif. 94122"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert B.","family":"Helling","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of California of San Francisco, San Francisco, Calif. 94122"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.11.3240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:32:38Z","timestamp":1649845958000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.11.3240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1973,11]]}},"alternative-id":["10.1073\/pnas.70.11.3240"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.11.3240","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,11]]},"assertion":[{"value":"1973-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}