{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:24:17Z","timestamp":1781105057549,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,12]]},"abstract":"<jats:p>\n                    Anaerobic \u03b2-galactoside transport in whole cells and membrane vesicles from\n                    <jats:italic>E. coli<\/jats:italic>\n                    ML 308-225 is coupled to the oxidation of \u03b1-glycerol-\n                    <jats:italic>P<\/jats:italic>\n                    or D-lactate with fumarate as an electron acceptor. Alternatively, anaerobic \u03b2-galactoside transport may be coupled to the oxidation of formate utilizing nitrate as electron acceptor. Both anaerobic electron-transfer systems are induced by growth of the organisms under appropriate conditions. Components of both systems are loosely bound to the membrane, necessitating the use of a modified procedure for vesicle preparation in order to demonstrate anaerobic transport\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Addition of ATP or an ATP-generating system to vesicles prepared from anaerobically-grown cells or inclusion of ATP or the ATP-generating system during preparation of vesicles does not stimulate transport. The results support the conclusion that active transport under anaerobic conditions is coupled primarily to electron flow.\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.12.3376","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:40:38Z","timestamp":1149043238000},"page":"3376-3381","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":78,"title":["Anaerobic Transport in\n                    <i>Escherichia coli<\/i>\n                    Membrane Vesicles"],"prefix":"10.1073","volume":"70","author":[{"given":"Wilhelmus N.","family":"Konings","sequence":"first","affiliation":[{"name":"The Laboratorium voor Microbiologie, University of Groningen, Kerklaan 30, Haren, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Ronald","family":"Kaback","sequence":"additional","affiliation":[{"name":"The Roche Institute of Molecular Biology, Nutley, New Jersey 07110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.12.3376","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:46:21Z","timestamp":1649846781000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.12.3376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1973,12]]}},"alternative-id":["10.1073\/pnas.70.12.3376"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.12.3376","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,12]]},"assertion":[{"value":"1973-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}