{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:29:13Z","timestamp":1781105353321,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,12]]},"abstract":"<jats:p>\n                    Purine metabolism has been examined in a clonal line of mouse neuroblastoma cells resistant to the cytotoxic effects of 6-thioguanine. Comparative studies in the resistant and parental lines indicate that the former cells have less than 1% of normal hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) activity. The activities of other enzymes important in the\n                    <jats:italic>de novo<\/jats:italic>\n                    and salvage pathways of purine biosynthesis were not significantly different in the two lines. Hypoxanthine phosphoribosyltransferase deficiency in this neuroblastoma line was associated with increased intracellular concentrations of 5-phosphoribosyl-1-pyrophosphate, an increased rate of purine biosynthesis\n                    <jats:italic>de novo<\/jats:italic>\n                    , and failure to incorporate hypoxanthine, but not adenine, into nucleotides. There are essentially the same alterations in purine metabolism that occur in hypoxanthine phosphoribosyltransferase-deficient fibroblasts or lymphoblasts derived from individuals with the Lesch-Nyhan syndrome. Clonal lines of hypoxanthine phosphoribosyltransferase-deficient neuroblastoma cells may therefore be of use in elucidating the mechanisms by which the enzyme defect leads to the neurologic dysfunction seen in children with this disease.\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.12.3880","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:40:38Z","timestamp":1149043238000},"page":"3880-3883","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":25,"title":["Purine Metabolism in Normal and Thioguanine-Resistant Neuroblastoma"],"prefix":"10.1073","volume":"70","author":[{"given":"Alexander W.","family":"Wood","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California, San Diego, La Jolla, Calif., 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Becker","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego, La Jolla, Calif., 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Minna","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Genetics, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland, 20014"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Edwin","family":"Seegmiller","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego, La Jolla, Calif., 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.12.3880","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:48:22Z","timestamp":1649846902000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.12.3880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1973,12]]}},"alternative-id":["10.1073\/pnas.70.12.3880"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.12.3880","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,12]]},"assertion":[{"value":"1973-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}