{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:16:59Z","timestamp":1791289019121,"version":"4.3.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,12]]},"abstract":"<jats:p>\n                    In tumors and embryoid bodies of mouse teratoma a correlation has been established between specific activity of alkaline phosphatase (EC 3.1.3.1) and content of embryonal carcinoma, the stem cell of the tumor. A histochemical study of embryoid bodies has shown that high levels of the enzyme are confined to embryonal carcinoma. Fifteen tissue culture lines could be classified into three groups: (\n                    <jats:italic>a<\/jats:italic>\n                    ) lines identifiable as pluripotential embryonal carcinoma by their morphology, tumorigenicity, and capacity to differentiate\n                    <jats:italic>in vivo<\/jats:italic>\n                    ; (\n                    <jats:italic>b<\/jats:italic>\n                    ) nullipotential embryonal carcinoma, resembling pluripotential embryonal carcinoma in morphology and malignancy but giving rise to undifferentiated tumors; and (\n                    <jats:italic>c<\/jats:italic>\n                    ) lines of apparently nonmalignant somatic cells. Both types of embryonal carcinoma possess levels of alkaline phosphatase 5- to a 100-fold higher than the somatic cell lines. The embryonal carcinoma enzyme resembles the enzymes from kidney and placenta in kinetics of thermal inactivation and sensitivity to the inhibitor L-phenylalanine, but is distinguishable from the alkaline phosphatases of liver and intestine. These findings are discussed in relation to the use of teratoma for the study of cell differentiation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.12.3899","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:40:38Z","timestamp":1149043238000},"page":"3899-3903","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":366,"title":["Alkaline Phosphatase Activity in Mouse Teratoma"],"prefix":"10.1073","volume":"70","author":[{"given":"Edward G.","family":"Bernstine","sequence":"first","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire du CNRS, 91190 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin L.","family":"Hooper","sequence":"additional","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire du CNRS, 91190 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Grandchamp","sequence":"additional","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire du CNRS, 91190 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Ephrussi","sequence":"additional","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire du CNRS, 91190 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,12]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.12.3899","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:48:46Z","timestamp":1649846926000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.12.3899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1973,12]]}},"alternative-id":["10.1073\/pnas.70.12.3899"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.12.3899","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,12]]},"assertion":[{"value":"1973-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}