{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:37:27Z","timestamp":1781109447704,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,3]]},"abstract":"<jats:p>\n                    Congenital neutropenia is a lethal disease characterized by recurrent infections beginning in the neonatal period, absence of neutrophils in the peripheral blood, eosinophilia, and monocytosis. The bone marrow shows an apparent \u201cmaturation arrest\u201d of the neutrophil series at the promyelocyte stage. Granulocytic colonies grown\n                    <jats:italic>in vitro<\/jats:italic>\n                    in soft agar medium show normal development of eosinophilic colonies and monocyte-macrophage colonies, but defective neutrophil maturation. The abnormal colonies observed contained only myeloblasts and promyelocytes. Thus, it seems to have been possible to mimic\n                    <jats:italic>in vitro<\/jats:italic>\n                    the abnormal differentiation that is observed\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.3.669","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:42:45Z","timestamp":1149043365000},"page":"669-672","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":23,"title":["Congenital Neutropenia:\n                    <i>In Vitro<\/i>\n                    Growth of Colonies Mimicking the Disease"],"prefix":"10.1073","volume":"70","author":[{"given":"Pierre","family":"L'Esperance","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Minnesota Medical School, Minneapolis, Minn. 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Brunning","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of Minnesota Medical School, Minneapolis, Minn. 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Good","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Minnesota Medical School, Minneapolis, Minn. 55455"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.3.669","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:40:05Z","timestamp":1649846405000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.3.669"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1973,3]]}},"alternative-id":["10.1073\/pnas.70.3.669"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.3.669","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,3]]},"assertion":[{"value":"1973-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}