{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:06:34Z","timestamp":1781103994011,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,4]]},"abstract":"<jats:p>\n                    <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>\n                    contains a factor that can replace adenosine 3\u2032:5\u2032-cyclic monophosphate (cAMP) in the stimulation of rabbit-muscle protein kinase. The factor cochromatographs and coelectrophoreses with authentic cAMP, and is inactivated by beef heart cyclic nucleotide phosphodiesterase. When\n                    <jats:italic>C. reinhardtii<\/jats:italic>\n                    is exposed to aminophylline (theophylline\n                    <jats:sub>2<\/jats:sub>\n                    ethylenediamine), the concentration of the factor in the cells increases within 1 hr, from about 25 pmol of cAMP equivalents per g dry weight to more than 250 pmol. Cyclic nucleotide phosphodiesterase activity is present in crude extract of\n                    <jats:italic>C. reinhardtii<\/jats:italic>\n                    and is inhibited by theophylline. We conclude that cAMP occurs in\n                    <jats:italic>C. reinhardtii<\/jats:italic>\n                    and that the endogenous concentration is governed at least in part by a theophylline-sensitive cyclic nucleotide phosphodiesterase. These findings provide a sound basis for attributing the effects of methylxanthines on flagellar function and regeneration in\n                    <jats:italic>C. reinhardtii<\/jats:italic>\n                    to the resultant elevation of endogenous cAMP.\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.4.1099","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:42:56Z","timestamp":1149043376000},"page":"1099-1103","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":53,"title":["Adenosine 3\u2032:5\u2032-Cyclic Monophosphate in\n                    <i>Chlamydomonas reinhardtii:<\/i>\n                    Isolation and Characterization"],"prefix":"10.1073","volume":"70","author":[{"given":"Nikolaus","family":"Amrhein","sequence":"first","affiliation":[{"name":"MSU\/AEC Plant Research Laboratory, Michigan State University, East Lansing, Mich. 48823"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Filner","sequence":"additional","affiliation":[{"name":"MSU\/AEC Plant Research Laboratory, Michigan State University, East Lansing, Mich. 48823"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.4.1099","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:46:35Z","timestamp":1649846795000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.4.1099"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1973,4]]}},"alternative-id":["10.1073\/pnas.70.4.1099"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.4.1099","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,4]]},"assertion":[{"value":"1973-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}