{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:03:05Z","timestamp":1781107385391,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,4]]},"abstract":"<jats:p>\n                    Discrimination between the physiological cleavage fragments of fibrinogen and fibrin offers an approach to differentiation between fibrinogenolytic processes and fibrinolysis after coagulation. By use of the cleavage-associated neoantigen of fibrinogen (fg-D\n                    <jats:sub>neo<\/jats:sub>\n                    ) as a molecular marker, characteristic differences between the D regions of fibrinogen derivatives and fibrin derivatives can be demonstrated. The expression of fg-D\n                    <jats:sub>neo<\/jats:sub>\n                    by X, Y, D:E complex, and D-fragments of fibrinogen or fibrin is shown to be quantitative and unitary. Characteristic differences between fg-D\n                    <jats:sub>neo<\/jats:sub>\n                    sites present on fibrinogen cleavage fragments, as contrasted to fibrin cleavage fragments, are indicated by different competitive inhibition slopes, and appear to reflect differential binding affinity of selected anti-fg-D\n                    <jats:sub>neo<\/jats:sub>\n                    antibodies for the specific molecular site. There is a linear relationship between the slope of quantitative competitive inhibition and the relative molar ratio of fibrinogen and fibrin derivatives. Identical immunochemical expressions are observed\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , and support the thesis that cleavage\n                    <jats:italic>in vivo<\/jats:italic>\n                    is produced by plasmin. The differential immunochemical features of fg-D\n                    <jats:sub>neo<\/jats:sub>\n                    expression may be the result of stable conformational and\/or subtle structural differences between the D region of fibrinogen and fibrin cleavage fragments and suggest that precise changes in the D region are associated with the fibrin transition. These molecular features not only provide additional insight into the molecular immunology and structure of fibrinogen, but also appear to offer a new molecular approach to discrimination between fibrinogenolytic mechanisms as contrasted to fibrinolysis secondary to coagulation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.4.1169","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:42:56Z","timestamp":1149043376000},"page":"1169-1173","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":13,"title":["Discriminating Neoantigenic Differences Between Fibrinogen and Fibrin Derivatives"],"prefix":"10.1073","volume":"70","author":[{"given":"Edward F.","family":"Plow","sequence":"first","affiliation":[{"name":"Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Edgington","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,4]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.4.1169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:46:27Z","timestamp":1649846787000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.4.1169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1973,4]]}},"alternative-id":["10.1073\/pnas.70.4.1169"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.4.1169","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,4]]},"assertion":[{"value":"1973-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}