{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:19:04Z","timestamp":1781104744310,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1973,7]]},"abstract":"<jats:p>\n                    Synthetic \u03b1T1 and \u03b2T1, the N-terminal tryptic peptides of \u03b1-chain and \u03b2-chain of hemoglobin, and Met\u03b1T1 and Met\u03b2T1, peptides in which N-terminal methionyl residues are peptide-bonded to \u03b1T1 and \u03b2T1, were prepared by the solid-state method of Merrifield. These synthetic peptides were used to establish conditions for chromatographic purification and analysis. When tryptic digests of nascent globin chains from rabbit and sickle-cell anemia reticulocytes incubated with\n                    <jats:sup>35<\/jats:sup>\n                    S- and\n                    <jats:sup>3<\/jats:sup>\n                    H-labeled amino acids were analyzed, radioactivity not present in tryptic digests of labeled hemoglobin appeared at the elution positions of Met\u03b1T1 and Met\u03b2T1. The fraction of nascent chains with N-terminal methionine was higher in sickle-cell anemia reticulocytes than in rabbit reticulocytes. If rate of peptide-chain elongation in polysomes is uniform, nascent human chains must attain a greater length before removal of the initial methionyl residue. Length of nascent chain at time of removal was calculated from two independent sets of data, one obtained from [\n                    <jats:sup>35<\/jats:sup>\n                    S]methionine incorporation into Met\u03b1T1, Met\u03b2T1, \u03b1T5, and \u03b2T5, and the other obtained from [\n                    <jats:sup>3<\/jats:sup>\n                    H]lysine and [\n                    <jats:sup>3<\/jats:sup>\n                    H]valine incorporation into Met\u03b2T1 and \u03b2T1.\n                  <\/jats:p>","DOI":"10.1073\/pnas.70.7.2059","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:40:11Z","timestamp":1149043211000},"page":"2059-2063","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":3,"title":["Delayed Removal of N-Terminal Methionine from Nascent Globin Chains in Sickle-Cell Anemia Reticulocytes"],"prefix":"10.1073","volume":"70","author":[{"given":"Haruo","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Pathology, University of California, San Diego, La Jolla, Calif. 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harvey A.","family":"Itano","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California, San Diego, La Jolla, Calif. 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1973,7]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.70.7.2059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:44:09Z","timestamp":1649846649000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.70.7.2059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1973,7]]}},"alternative-id":["10.1073\/pnas.70.7.2059"],"URL":"https:\/\/doi.org\/10.1073\/pnas.70.7.2059","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,7]]},"assertion":[{"value":"1973-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}