{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:25:59Z","timestamp":1781105159100,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1974,3]]},"abstract":"<jats:p>A brilliant, coarsely granular nuclear antigen was detected by anti-complement immunofluorescence in the nuclei of acute myeloid leukemia myeloblasts. Designated as LANA (leukemia-associated nuclear antigen), the reactivity differs from that of the Epstein-Barr-virus-determined nuclear antigen (EBNA) in immunological specificity and morphological appearance, although it is visualized by the same method. Serum from acute myeloid leukemia patients gave positive reactions in 73% of the cases. In acute lymphatic leukemia, chronic myeloid leukemia, chronic lymphatic leukemia, and Burkitt's lymphoma the sera were positive in 35, 14, 19, and 24%, respectively. Two of five polycythemia and two of eleven myeloma sera were also positive. Among 61 healthy controls, 58 were negative, whereas three showed a diffuse nuclear staining with a different pattern. Among 24 carcinoma patients, 18 were negative, whereas six gave a nuclear staining with a different, diffuse pattern. Sera from 20 patients who had recovered from infectious mononucleosis were all negative. In addition to the blasts of acute myeloid leukemia, a similar reactivity was seen with two Epstein-Barr virus DNA and EBNA-negative African lymphoma biopsies and in a short-lived tissue culture line derived from one of them. LANA could be a fetal or tissue-specific antigen, a virally determined antigen, or a specific form of anti-nuclear reactivity.<\/jats:p>","DOI":"10.1073\/pnas.71.3.685","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:53:47Z","timestamp":1149044027000},"page":"685-689","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["Human Leukemia-Associated Anti-Nuclear Reactivity"],"prefix":"10.1073","volume":"71","author":[{"given":"George","family":"Klein","sequence":"first","affiliation":[{"name":"Department of Tumor Biology, Karolinska Institutet, S 104 01 Stockholm 60, Sweden"},{"name":"Department of Immunology, Hadassah Medical School, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melita","family":"Steiner","sequence":"additional","affiliation":[{"name":"Department of Tumor Biology, Karolinska Institutet, S 104 01 Stockholm 60, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Wiener","sequence":"additional","affiliation":[{"name":"Department of Tumor Biology, Karolinska Institutet, S 104 01 Stockholm 60, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Tumor Biology, Karolinska Institutet, S 104 01 Stockholm 60, Sweden"},{"name":"Department of Immunology, Hadassah Medical School, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1974,3]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.71.3.685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:38:22Z","timestamp":1649846302000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.71.3.685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1974,3]]}},"alternative-id":["10.1073\/pnas.71.3.685"],"URL":"https:\/\/doi.org\/10.1073\/pnas.71.3.685","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,3]]},"assertion":[{"value":"1974-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}