{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:57:14Z","timestamp":1781110634030,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1974,5]]},"abstract":"<jats:p>\n                    Binding sites with high affinity and specificity for [\n                    <jats:sup>3<\/jats:sup>\n                    H]quinuclidinyl benzilate (QNB) are present in homogenates of rat brain. The characteristics of the binding sites resemble those of muscarinic cholinergic receptors. Specific binding is saturable with respect to [\n                    <jats:sup>3<\/jats:sup>\n                    H]QNB and tissue concentration and is time-, temperature-, and pH-dependent. The bimolecular rate of association (2.0 \u00d7 10\n                    <jats:sup>8<\/jats:sup>\n                    M\n                    <jats:sup>-1<\/jats:sup>\n                    min\n                    <jats:sup>-1<\/jats:sup>\n                    ) and dissociation (1.2 \u00d7 10\n                    <jats:sup>-2<\/jats:sup>\n                    min\n                    <jats:sup>-1<\/jats:sup>\n                    ) at 35\u00b0 indicate a dissociation constant of 60 pM and a density of 65 pmol\/g of brain. Muscarinic antagonists and agonists displace specific [\n                    <jats:sup>3<\/jats:sup>\n                    H]QNB binding, while nicotinic and non-cholinergic drugs possess little affinity for [\n                    <jats:sup>3<\/jats:sup>\n                    H]QNB-binding sites.\n                  <\/jats:p>","DOI":"10.1073\/pnas.71.5.1725","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:55:31Z","timestamp":1149044131000},"page":"1725-1729","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":636,"title":["Muscarinic Cholinergic Binding in Rat Brain"],"prefix":"10.1073","volume":"71","author":[{"given":"Henry I.","family":"Yamamura","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Experimental Therapeutics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solomon H.","family":"Snyder","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Experimental Therapeutics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1974,5]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.71.5.1725","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:23:58Z","timestamp":1649845438000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.71.5.1725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1974,5]]}},"alternative-id":["10.1073\/pnas.71.5.1725"],"URL":"https:\/\/doi.org\/10.1073\/pnas.71.5.1725","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,5]]},"assertion":[{"value":"1974-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}