{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:28:32Z","timestamp":1781105312433,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1974,5]]},"abstract":"<jats:p>The influence of autonomic neurohormones on the immunologic release of \u03b2-glucuronidase (EC 3.2.1.31) from, and the cyclic nucleotide levels in, human neutrophils was determined. Interaction of neutrophils with rheumatoid arthritic, serum-treated zymosan particles in a neutral balanced salt solution at 37\u00b0 resulted in the extracellular discharge of \u03b2-glucuronidase without any loss of cell viability, as indicated by the failure of incubated cells to take up eosin Y or to release cytoplasmic lactate dehydrogenase (EC 1.1.1.27). Epinephrine reduced the release of \u03b2-glucuronidase from neutrophils in the presence of zymosan during 2-30 min of incubation and elicited a concomitant elevation of adenosine 3\u2032:5\u2032-monophosphate levels. Propranolol, a \u03b2-adrenergic receptor antagonist, but not phentolamine, an \u03b1-adrenergic receptor antagonist, blocked both actions of epinephrine. Acetylcholine stimulated the release of \u03b2-glucuronidase, but not lactate dehydrogenase, and provoked a concomitant elevation of guanosine 3\u2032:5\u2032-monophosphate levels. Atropine, a muscarinic receptor antagonist, but not hexamethonium, a ganglionic blocker, inhibited both actions of acetylcholine. Interaction of neutrophils and zymosan particles resulted in an elevation of guanosine 3\u2032:5\u2032-monophosphate levels within 2 min. These data suggest that intracellular guanosine 3\u2032:5\u2032-monophosphate may be involved in mediating the immunologic release of lysosomal enzymes from human neutrophils whereas adenosine 3\u2032:5\u2032-monophosphate may inhibit enzyme release. Moreover, autonomic neurohormones appear to be capable of modulating lysosomal enzyme release by virtue of their capacity to elevate neutrophil cyclic nucleotide levels.<\/jats:p>","DOI":"10.1073\/pnas.71.5.2027","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:55:31Z","timestamp":1149044131000},"page":"2027-2031","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":61,"title":["Hormonal Control of Lysosomal Enzyme Release from Human Neutrophils: Elevation of Cyclic Nucleotide Levels by Autonomic Neurohormones"],"prefix":"10.1073","volume":"71","author":[{"given":"Louis J.","family":"Ignarro","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"George","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1974,5]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.71.5.2027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:24:40Z","timestamp":1649845480000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.71.5.2027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1974,5]]}},"alternative-id":["10.1073\/pnas.71.5.2027"],"URL":"https:\/\/doi.org\/10.1073\/pnas.71.5.2027","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,5]]},"assertion":[{"value":"1974-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}