{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:19:29Z","timestamp":1792192769210,"version":"4.4.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1974,6]]},"abstract":"<jats:p>\n                    Interaction of 21 benzodiazepines with the glycine receptor in the brainstem and spinal cord of rat have been evaluated in terms of their displacement of [\n                    <jats:sup>3<\/jats:sup>\n                    H]strychinine binding. The rank order of potency of the 21 drugs in displacing specific [\n                    <jats:sup>3<\/jats:sup>\n                    H]strychinine binding correlates (p &lt; 0.005) with their rank order of potency in a vareity of pharmacological and behavioral tests in humans and animals that predict clinical efficacy. There is a 50-fold variation in potency of the series of benzodiazepines with mean effective dose (ED\n                    <jats:sub>50<\/jats:sub>\n                    ) values ranging from 19\u03bcM to &gt; 1000 \u03bcM. Diazepam (Valium\u00ae) and chlordiazepoxide (Librium\u00ae) have ED\n                    <jats:sub>50<\/jats:sub>\n                    's of 26 \u03bcM and 200 \u03bcM, respectively, whereas the ED\n                    <jats:sub>50<\/jats:sub>\n                    for glycine is 25 \u03bcM. The inhibitory effects of 10 of the agents in two other central nervous system membrane receptor assays, for the opiate receptor and the muscarinic cholinergic receptor, do not correlate with any of the\n                    <jats:italic>in vivo<\/jats:italic>\n                    pharmacologic and behavioral tests. The benzodiazepines may exert their antianxiety, anticonvulsant and muscle-relaxant effects by mimicking the effects of the neurotransmitter glycine at its central nervous system receptor sites.\n                  <\/jats:p>","DOI":"10.1073\/pnas.71.6.2246","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:56:34Z","timestamp":1149044194000},"page":"2246-2250","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":99,"title":["Interaction of Benzodiazepines with Central Nervous Glycine Receptors: Possible Mechanism of Action"],"prefix":"10.1073","volume":"71","author":[{"given":"Anne B.","family":"Young","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Experimental Therapeutics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R.","family":"Zukin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Experimental Therapeutics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solomon H.","family":"Snyder","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Experimental Therapeutics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"},{"name":"Department of Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1974,6]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.71.6.2246","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:26:50Z","timestamp":1649845610000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.71.6.2246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1974,6]]}},"alternative-id":["10.1073\/pnas.71.6.2246"],"URL":"https:\/\/doi.org\/10.1073\/pnas.71.6.2246","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,6]]},"assertion":[{"value":"1974-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}