{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:55:04Z","timestamp":1781106904125,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1974,6]]},"abstract":"<jats:p>\n                    1\u03b1,25-dihydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    was examined for its ability to affect the DNA-dependent RNA polymerases (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of rachitic chick intestinal cell nuclei\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Nucleoplasmic (form II) RNA polymerase activity was stimulated 2-fold (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) within 2-3 hr after an oral dose of 0.27 \u03bcg (0.65 nmol) of 1\u03b1,25-dihydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    to rachitic chicks. The form II polymerase activity returned to control values by 5-9 hr after dosing with the sterol. In contrast, the nucleolar (form I) RNA polymerase was not increased within this period. Solubilization of nuclear protein and resolution of the two RNA polymerases on DEAE-Sephadex also revealed that there was an increase in polymerase II activity when assayed on exogenous DNA template. This evidence suggests that 1\u03b1,25-dihydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    acts at the level of the enzymology of intestinal cell transcription and that increased mRNA synthesis after administration of this hormone cannot be due solely to a change in chromatin template activity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.71.6.2337","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:56:34Z","timestamp":1149044194000},"page":"2337-2341","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":40,"title":["Rapid Enhancement of Chick Intestinal DNA-Dependent RNA Polymerase II Activity by 1\u03b1,25-Dihydroxyvitamin D\n                    <sub>3<\/sub>\n                    ,\n                    <i>In Vivo<\/i>"],"prefix":"10.1073","volume":"71","author":[{"given":"Joseph E.","family":"Zerwekh","sequence":"first","affiliation":[{"name":"Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Ariz. 85724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Haussler","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Ariz. 85724"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Lindell","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Medicine, University of Arizona, Tucson, Ariz. 85724"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1974,6]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.71.6.2337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:27:05Z","timestamp":1649845625000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.71.6.2337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1974,6]]}},"alternative-id":["10.1073\/pnas.71.6.2337"],"URL":"https:\/\/doi.org\/10.1073\/pnas.71.6.2337","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,6]]},"assertion":[{"value":"1974-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}