{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:21:33Z","timestamp":1781104893958,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1974,9]]},"abstract":"<jats:p>\n                    The microsomal fraction of bovine vesicular gland catalyzed the conversion of [\n                    <jats:italic>1<\/jats:italic>\n                    -\n                    <jats:sup>14<\/jats:sup>\n                    C]8,11,14-eicosatrienoic acid to prostaglandin E\n                    <jats:sub>1<\/jats:sub>\n                    in the presence of tryptophan, hemoglobin, and glutathione. The prostaglandin synthetase system was solubilized by treatment of the microsomal fraction with Tween 20 in the presence of ethylene glycol. DEAE-cellulose column chromatography separated the enzyme into two fractions (Fractions I and II), both of which were required for prostaglandin E\n                    <jats:sub>1<\/jats:sub>\n                    synthesis. When Fraction I alone was incubated with 8,11,14-eicosatrienoic acid, an unstable compound accumulated. This compound was converted to prostaglandin E\n                    <jats:sub>1<\/jats:sub>\n                    by the addition of Fraction II. On the basis of its\n                    <jats:italic>\n                      R\n                      <jats:sub>F<\/jats:sub>\n                    <\/jats:italic>\n                    values on thin-layer chromatography, its reduction to prostaglandin F\n                    <jats:sub>1\u03b1<\/jats:sub>\n                    with stannous chloride, and the decomposition to prostaglandins F\n                    <jats:sub>1\u03b1<\/jats:sub>\n                    , E\n                    <jats:sub>1<\/jats:sub>\n                    , and D\n                    <jats:sub>1<\/jats:sub>\n                    at room temperature, the unstable intermediate was tentatively identified to be the 9,11-endoperoxide derivative, referred to previously as prostaglandin R\n                    <jats:sub>1<\/jats:sub>\n                    (Nugteren\n                    <jats:italic>et al.<\/jats:italic>\n                    (1973)\n                    <jats:italic>Biochim. Biophys. Acta<\/jats:italic>\n                    326, 448-461) or prostaglandin H\n                    <jats:sub>1<\/jats:sub>\n                    (Hamberg\n                    <jats:italic>et al.<\/jats:italic>\n                    (1974)\n                    <jats:italic>Proc. Nat. Acad. Sci. USA<\/jats:italic>\n                    71, 345-349). Of the three cofactors mentioned above, Fraction I required both tryptophan and hemoglobin, while only glutathione was necessary for Fraction II. Anti-inflammatory agents such as indomethacin and aspirin were inhibitory to Fraction I.\n                  <\/jats:p>","DOI":"10.1073\/pnas.71.9.3645","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T02:59:14Z","timestamp":1149044354000},"page":"3645-3648","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":77,"title":["Prostaglandin Synthetase System\u2014Resolution into Oxygenase and Isomerase Components"],"prefix":"10.1073","volume":"71","author":[{"given":"Tsumoru","family":"Miyamoto","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shozo","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Hayaishi","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Kyoto University Faculty of Medicine, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1974,9]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.71.9.3645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:47:59Z","timestamp":1649846879000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.71.9.3645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1974,9]]}},"alternative-id":["10.1073\/pnas.71.9.3645"],"URL":"https:\/\/doi.org\/10.1073\/pnas.71.9.3645","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,9]]},"assertion":[{"value":"1974-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}