{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:27:21Z","timestamp":1790854041311,"version":"4.1.0"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1977,11]]},"abstract":"<jats:p>Cyclic GMP-dependent protein kinase, purified to homogeneity from bovine lung, was shown to activate hormone-sensitive lipase partially purified from chicken adipose tissue. The degree of activation was the same as that effected by cyclic AMP-dependent protein kinase although higher concentrations of the cyclic GMP-dependent enzyme were required (relative activities expressed in terms of histone H2b phosphorylation units). Activation by cyclic AMP-dependent protein kinase was completely blocked by the heat-stable protein kinase inhibitor protein from skeletal muscle but activation by the cyclic GMP enzyme was not inhibited. Lipase fully activated by cyclic AMP-dependent protein kinase showed no further change in activity when treated with cyclic GMP-dependent protein kinase. Lipase activated by cyclic GMP-dependent protein kinase was reversibly deactivated by purified phosphorylase phosphatase (from bovine heart); full activity was restored by reincubation with cyclic GMP and cyclic GMP-dependent protein kinase. Cholesterol esterase activity in the chicken adipose tissue fraction, previously shown to be activated along with the triglyceride lipase by cyclic AMP-dependent protein kinase, was also activated by cyclic GMP-dependent protein kinase. Crude preparations of hormone-sensitive triglyceride lipase from human or rat adipose tissue and cholesterol esterase from rat adrenal were also activated by cyclic GMP-dependent protein kinase. Purified phosphorylase kinase (rabbit skeletal muscle) was also shown to be activated by cyclic GMP-dependent protein kinase. The present results, together with those of other workers on histone phosphorylation, suggest that the substrate specificities of cyclic GMP-dependent and cyclic AMP-dependent protein kinase may be similar. This is discussed in the light of a model recently proposed with regard to the relationship between the subunit structures of the two kinases. The physiologic significance of the findings remains to be established.<\/jats:p>","DOI":"10.1073\/pnas.74.11.4843","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T03:24:55Z","timestamp":1149045895000},"page":"4843-4847","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":51,"title":["Activation of hormone-sensitive lipase and phosphorylase kinase by purified cyclic GMP-dependent protein kinase"],"prefix":"10.1073","volume":"74","author":[{"given":"John C.","family":"Khoo","sequence":"first","affiliation":[{"name":"Division of Metabolic Disease, University of California, San Diego, La Jolla, California 92093"},{"name":"Division of Endocrinology, Department of Medicine, University of California, San Diego, La Jolla, California 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela J.","family":"Sperry","sequence":"additional","affiliation":[{"name":"Division of Metabolic Disease, University of California, San Diego, La Jolla, California 92093"},{"name":"Division of Endocrinology, Department of Medicine, University of California, San Diego, La Jolla, California 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon N.","family":"Gill","sequence":"additional","affiliation":[{"name":"Division of Metabolic Disease, University of California, San Diego, La Jolla, California 92093"},{"name":"Division of Endocrinology, Department of Medicine, University of California, San Diego, La Jolla, California 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Steinberg","sequence":"additional","affiliation":[{"name":"Division of Metabolic Disease, University of California, San Diego, La Jolla, California 92093"},{"name":"Division of Endocrinology, Department of Medicine, University of California, San Diego, La Jolla, California 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1977,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.74.11.4843","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:10:21Z","timestamp":1649848221000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.74.11.4843"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1977,11]]}},"alternative-id":["10.1073\/pnas.74.11.4843"],"URL":"https:\/\/doi.org\/10.1073\/pnas.74.11.4843","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,11]]},"assertion":[{"value":"1977-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}