{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:21:54Z","timestamp":1781108514299,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1977,11]]},"abstract":"<jats:p>\n                    Dextran was used to trace membrane retrieved from the luminal surface after induced exocytosis in secretory cells of rat lacrimal and parotid glands. Two different approaches were used: (\n                    <jats:italic>a<\/jats:italic>\n                    ) isolated acini were incubated\n                    <jats:italic>in vitro<\/jats:italic>\n                    with dextran followed by stimulation with carbamylcholine (lacrimal) or isoproterenol (parotid) and (\n                    <jats:italic>b<\/jats:italic>\n                    ) rats were injected with isoproterenol followed by dextran infusion into the parotid duct\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The main findings were the same regardless of the gland source or experimental approach. Dextran was taken up initially via coated pits into smooth-surfaced apical vesicles. Shortly thereafter it was found in multiple cell compartments: within the stacked Golgi cisternae, in condensing vacuoles, and in lysosomes. Uptake was more rapid and uniform\n                    <jats:italic>in vivo<\/jats:italic>\n                    ; dextran was seen in multiple cisternae of numerous Golgi complexes within 5 min after infusion. In acini incubated\n                    <jats:italic>in vitro<\/jats:italic>\n                    uptake into Golgi cisternae was more delayed and occurred with increasing frequency up to 60 min; also, more dextran was taken up into lysosomes, which were more numerous\n                    <jats:italic>in vitro<\/jats:italic>\n                    than\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The results demonstrate that, after exocytosis, membrane is removed from the cell surface via vesicles that fuse with multiple cell compartments. The two novel findings are: (\n                    <jats:italic>a<\/jats:italic>\n                    ) the demonstration that the tracer can reach most of the Golgi cisternae in a given stack and (\n                    <jats:italic>b<\/jats:italic>\n                    ) the demonstration of the rapidity with which the process takes place (i.e., within 5 min). The findings imply that at least some membrane retrieved from the cell surface after exocytosis fuses with the stacked Golgi cisternae.\n                  <\/jats:p>","DOI":"10.1073\/pnas.74.11.5073","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T03:24:55Z","timestamp":1149045895000},"page":"5073-5077","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":120,"title":["Luminal membrane retrieved after exocytosis reaches most Golgi cisternae in secretory cells"],"prefix":"10.1073","volume":"74","author":[{"given":"Volker","family":"Herzog","sequence":"first","affiliation":[{"name":"Department of Cell Biology, University of Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilyn G.","family":"Farquhar","sequence":"additional","affiliation":[{"name":"Section of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1977,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.74.11.5073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:10:53Z","timestamp":1649848253000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.74.11.5073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1977,11]]}},"alternative-id":["10.1073\/pnas.74.11.5073"],"URL":"https:\/\/doi.org\/10.1073\/pnas.74.11.5073","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,11]]},"assertion":[{"value":"1977-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}