{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:53:56Z","timestamp":1781103236393,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1978,11]]},"abstract":"<jats:p>Xenpus laevis oocytes over a size range of 0.15--0.78 mm3 were dissected from their follicles and cultured in a defined medium for up to 28 days. Oocytes grew at average rates of 0.021 mm3.day-1 in the absence of insulin and 0.030 mm3.day-1 in the presence of insulin. The latter average growth rate corresponds to the fastest growth rate reported to date for oocytes in vivo. Oocytes grown in vitro can reach a size of at least 1.43 mm3, which is larger than the maximum size generally found in vivo. During growth in vitro; oocytes also acquire both a normal pigment pattern and, once they reach about 0.7 mm3, the ability to undergo complete maturation as a response to externally applied progesterone. These results show that Xenopus oocytes freed of their follicular investments are able to grow and differentiate in vitro.<\/jats:p>","DOI":"10.1073\/pnas.75.11.5534","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T03:38:12Z","timestamp":1149046692000},"page":"5534-5538","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":59,"title":["Long-term growth and differentiation of Xenopus oocytes in a defined medium."],"prefix":"10.1073","volume":"75","author":[{"given":"R A","family":"Wallace","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Misulovin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1978,11]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.75.11.5534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T11:02:14Z","timestamp":1649847734000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.75.11.5534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1978,11]]}},"alternative-id":["10.1073\/pnas.75.11.5534"],"URL":"https:\/\/doi.org\/10.1073\/pnas.75.11.5534","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,11]]},"assertion":[{"value":"1978-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}