{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:53:33Z","timestamp":1781103213115,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1978,5]]},"abstract":"<jats:p>\n                    Isolated rat hepatocytes do not actively accumulate Ca\n                    <jats:sup>2+<\/jats:sup>\n                    during prolonged incubation\n                    <jats:italic>in vitro<\/jats:italic>\n                    ; however, these cells do exhibit a limited exchange of intracellular with extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . The exchangeable pool represents about 2 nmol of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    per mg of protein. In medium containing either a low (20 \u03bcM) or high (1 mM) concentration of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , the divalent cation ionophore, A23187 (at concentrations of 0.03-0.1 nmol\/mg of protein), causes release of\n                    <jats:sup>45<\/jats:sup>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    from this exchangeable pool but does not allow net influx of extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    detectable by the use of a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -sensitive electrode. Like A23187, the hormones norepinephrine, vasopressin, and glucagon (at concentrations that stimulate gluconeogenesis) each induces a similar net efflux of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . Treatment with one hormone decreases the subsequent reponse to the others, whereas treatment with A23187 abolishes the hormonal effects upon both Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release and gluconeogenesis. The action of norepinephrine, but not of glucagon, upon Ca\n                    <jats:sup>2+<\/jats:sup>\n                    efflux is prevented by the \u03b1-adrenergic antagonist, phenoxybenzamine. The action of norepinephrine is not prevented by the \u03b2-adrenergic antagonist, propranolol. Together these results indicate that the release of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    from a common pool of exchangeable Ca\n                    <jats:sup>2+<\/jats:sup>\n                    is important to the action of a variety of hormones on hepatocytes. This Ca\n                    <jats:sup>2+<\/jats:sup>\n                    pool in the isolated hepatocyte is characterized as being similar in size and having exchange kinetics that are comparable to those reported for the major intracellular pool of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    in the intact liver. The possibility that this pool is intramitochondrial calcium is discussed.\n                  <\/jats:p>","DOI":"10.1073\/pnas.75.5.2234","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T03:43:10Z","timestamp":1149046990000},"page":"2234-2238","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":107,"title":["Norepinephrine, vasopressin, glucagon, and A23187 induce efflux of calcium from an exchangeable pool in isolated rat hepatocytes"],"prefix":"10.1073","volume":"75","author":[{"given":"Jen-Ling J.","family":"Chen","sequence":"first","affiliation":[{"name":"The Institute for Enzyme Research, The University of Wisconsin, Madison, Wisconsin 53706"},{"name":"Department of Biochemistry, The University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donner F.","family":"Babcock","sequence":"additional","affiliation":[{"name":"The Institute for Enzyme Research, The University of Wisconsin, Madison, Wisconsin 53706"},{"name":"Department of Biochemistry, The University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry A.","family":"Lardy","sequence":"additional","affiliation":[{"name":"The Institute for Enzyme Research, The University of Wisconsin, Madison, Wisconsin 53706"},{"name":"Department of Biochemistry, The University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1978,5]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.75.5.2234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:49:43Z","timestamp":1649846983000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.75.5.2234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1978,5]]}},"alternative-id":["10.1073\/pnas.75.5.2234"],"URL":"https:\/\/doi.org\/10.1073\/pnas.75.5.2234","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,5]]},"assertion":[{"value":"1978-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}