{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:49:02Z","timestamp":1781102942779,"version":"3.54.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1979,10]]},"abstract":"<jats:p>\n                    Poly(A)-containing RNA isolated from bovine and mouse pituitaries and a mouse pituitary thyrotropic tumor was translated in a wheat germ cell-free biosynthetic system. A precursor of the glycoprotein hormone \u03b1 subunit, \u201cpre-\u03b1,\u201d was immunoprecipitated from the translation mixtures with antiserum against bovine luteinizing hormone (LH; lutropin) \u03b1. The specificity of the immunoprecipitation was shown by competition with authentic bovine LH\u03b1 and lack of competition with bovine thyroid-stimulating hormone (TSH; thyrotropin) \u03b2. Bovine and mouse pre-\u03b1 subunits migrated identically in sodium dodecyl sulfate gradient polyacrylamide slab gels with an apparent molecular weight of about 17,000. Pre-\u03b1 comprised 2-3% and 20-30% of the total proteins translated with pituitary and pituitary tumor mRNA, respectively. Microanalysis of amino acid sequence of the pre-\u03b1 subunits containing various radiolabeled amino acids gave the following partial sequence for mouse tumor pre-\u03b1: [Formula: see text] Met was also found in positions 1, 14, and 17 in mouse pituitary pre-\u03b1 but only in residue 1 of the bovine pituitary pre-\u03b1 subunit. Leu was found in identical positions in bovine pituitary pre-\u03b1, with an additional Leu in position 17. Leu in the common positions (12, 15, 19, and 22) has also been found in human choriogonadotropin pre-\u03b1 subunit [Birken, S., Fetherston, J., Desmond, J., Canfield, R. &amp; Boime, I. (1978)\n                    <jats:italic>Biochem. Biophys. Res. Commun.<\/jats:italic>\n                    85, 1247-1253]. The data demonstrate that pituitary as well as placental glycoprotein hormone \u03b1 subunits are synthesized with an amino-terminal hydrophobic extension, in accord with the \u201csignal hypothesis\u201d for secreted proteins. Furthermore, the positions of the hydrophobic amino acid Leu have been strictly conserved in pre-\u03b1 subunits from various species and in two different tissues, the pituitary and placenta.\n                  <\/jats:p>","DOI":"10.1073\/pnas.76.10.4798","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T03:51:44Z","timestamp":1149047504000},"page":"4798-4802","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":19,"title":["Comparison of bovine and mouse pituitary glycoprotein hormone pre-\u03b1 subunits synthesized\n                    <i>in vitro<\/i>"],"prefix":"10.1073","volume":"76","author":[{"given":"Linda C.","family":"Giudice","sequence":"first","affiliation":[{"name":"Clinical Endocrinology Branch, National Institute of Arthrits, Metabolism, and Digestive Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myron J.","family":"Waxdal","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, National Institute of Allergic and Infectious Diseases, Bethesda, Maryland 20205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce D.","family":"Weintraub","sequence":"additional","affiliation":[{"name":"Clinical Endocrinology Branch, National Institute of Arthrits, Metabolism, and Digestive Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1979,10]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.76.10.4798","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T10:52:23Z","timestamp":1649847143000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.76.10.4798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1979,10]]}},"alternative-id":["10.1073\/pnas.76.10.4798"],"URL":"https:\/\/doi.org\/10.1073\/pnas.76.10.4798","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,10]]},"assertion":[{"value":"1979-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}